Skip Navigation



JXB Advance Access published online on September 10, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh252
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/406/2251    most recent
erh252v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Yang, X.
Right arrow Articles by Weston, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, X.
Right arrow Articles by Weston, L. A.
Agricola
Right arrow Articles by Yang, X.
Right arrow Articles by Weston, L. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received March 12, 2004
Accepted July 16, 2004

RESEARCH PAPER

SOR1, a gene associated with bioherbicide production in sorghum root hairs

Xiaohan Yang 1, Brian E. Scheffler 2, and Leslie A. Weston 1*

1 Department of Horticulture, Cornell University, Ithaca, NY 14853, USA
2 USDA-ARS-NPURU, National Center for Natural Products Research, Oxford, MS 38677, USA; USDA-ARS-CGRU, MSA Genomics Laboratory, 141 Experiment Station Rd., Stoneville, MS 38776, USA

* To whom correspondence should be addressed. E-mail: law20{at}cornell.edu.


  Abstract

Sorghum [Sorghum bicolor (L.) Moench] roots exude a potent bioherbicide known as sorgoleone, which is produced in living root hairs and is phytotoxic to broadleaf and grass weeds at concentrations as low as 10 µM. Differential gene expression was studied in sorghum (S. bicolorxS. sudanense) cv. SX17 between roots with abundant root hairs and those without root hairs using a modified differential display approach. A differentially expressed gene, named SOR1, was cloned by using Rapid Amplification of the 5' ends of cDNA (5'-RACE). Real-time PCR analysis of multiple tissues of sorghum SX17 revealed that the SOR1 transcript level in root hairs was more than 1000 times higher than that of other tissues evaluated, including immature leaf, mature leaf, mature stem, panicle, and roots with hairs removed. Semi-quantitative RT-PCR revealed that SOR1 was expressed in the sorgoleone-producing roots of sorghum SX17, shattercane [S. bicolor (L.) Moench], and johnsongrass [S. halepense (L.) Pers.], but not in the shoots of sorghum or in the roots of sweet corn (Zea mays L.) ‘Summer Flavor 64Y’, in which sorgoleone production was not detected by HPLC analysis. Similarity searches indicated that SOR1 probably encodes a novel desaturase, which might be involved in the formation of a unique and specific double bonding pattern within the long hydrocarbon tail of sorgoleone.

Keywords: Allelopathy; differential display; gene cloning; real-time PCR; root exudates; root hair; sorghum; sorgoleone.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. R. Baerson, F. E. Dayan, A. M. Rimando, N. P. D. Nanayakkara, C.-J. Liu, J. Schroder, M. Fishbein, Z. Pan, I. A. Kagan, L. H. Pratt, et al.
A Functional Genomics Investigation of Allelochemical Biosynthesis in Sorghum bicolor Root Hairs
J. Biol. Chem., February 8, 2008; 283(6): 3231 - 3247.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Z. Pan, A. M. Rimando, S. R. Baerson, M. Fishbein, and S. O. Duke
Functional Characterization of Desaturases Involved in the Formation of the Terminal Double Bond of an Unusual 16:3{Delta}9, 12, 15 Fatty Acid Isolated from Sorghum bicolor Root Hairs
J. Biol. Chem., February 16, 2007; 282(7): 4326 - 4335.
[Abstract] [Full Text] [PDF]

Home page
 Agron. J.Home page
S. Machado
Allelopathic Potential of Various Plant Species on Downy Brome: Implications for Weed Control in Wheat Production
Agron. J., January 1, 2007; 99(1): 127 - 132.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.