Skip Navigation



JXB Advance Access published online on October 8, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh253
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/408/2559    most recent
erh253v2
erh253v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by de Pinto, M. C.
Right arrow Articles by De Gara, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Pinto, M. C.
Right arrow Articles by De Gara, L.
Agricola
Right arrow Articles by de Pinto, M. C.
Right arrow Articles by De Gara, L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received April 8, 2004
Accepted July 16, 2004

RESEARCH PAPER

Changes in the ascorbate metabolism of apoplastic and symplastic spaces are associated with cell differentiation

Maria Concetta de Pinto 1 and Laura De Gara 2*

1 Dipartimento di Biologia e Patologia Vegetale, Università degli Studi di Bari, Via E. Orabona 4, I-70125 Bari, Italy
2 Dipartimento di Biologia e Patologia Vegetale, Università degli Studi di Bari, Via E. Orabona 4, I-70125 Bari, Italy; Interdisciplinary Center for Biomedical Research (CIR), Università Campus Biomedico, via Longoni 83, I-00155 Roma, Italy

* To whom correspondence should be addressed. E-mail: degara{at}botanica.uniba.it.


  Abstract

Ascorbate levels and redox state, as well as the activities of the ascorbate related enzymes, have been analysed both in the apoplastic and symplastic spaces of etiolated pea (Pisum sativum L.) shoots during cellular differentiation. The ascorbate pool and the ascorbate oxidizing enzymes, namely ascorbate oxidase and ascorbate peroxidase, were present in both pea apoplast and symplast, whereas ascorbate free radical reductase and dehydroascorbate reductase were only present in the symplastic fractions. During cell differentiation the ascorbate redox enzymes changed in different ways, since a decrease in ascorbate levels, ascorbate peroxidase and ascorbate free radical reductase occurred from meristematic to differentiated cells, whereas ascorbate oxidase and dehydroascorbate reductase increased. The activity of secretory peroxidases has also been followed in the apoplast of meristematic and differentiating cells. These peroxidases increased their activity during differentiation. This behaviour was accompanied by changes in their isoenzymatic profiles. The analysis of the kinetic characteristics of the different peroxidases present in the apoplast suggests that the presence of ascorbate and ascorbate peroxidase in the cell wall could play a critical role in regulating the wall stiffening process during cell differentiation by interfering with the activity of secretory peroxidases.

Keywords: Ascorbate; cell wall; class III peroxidases; dehydroascorbate; galactono-{gamma}-lactone dehydrogenase; plant cell differentiation; reactive oxygen species.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
V. Preger, N. Tango, C. Marchand, S. D. Lemaire, D. Carbonera, M. Di Valentin, A. Costa, P. Pupillo, and P. Trost
Auxin-Responsive Genes AIR12 Code for a New Family of Plasma Membrane b-Type Cytochromes Specific to Flowering Plants
Plant Physiology, June 1, 2009; 150(2): 606 - 620.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P Diaz-Vivancos, M Rubio, V Mesonero, P. Periago, A Ros Barcelo, P Martinez-Gomez, and J. Hernandez
The apoplastic antioxidant system in Prunus: response to long-term plum pox virus infection
J. Exp. Bot., November 1, 2006; 57(14): 3813 - 3824.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Pavet, E. Olmos, G. Kiddle, S. Mowla, S. Kumar, J. Antoniw, M. E. Alvarez, and C. H. Foyer
Ascorbic Acid Deficiency Activates Cell Death and Disease Resistance Responses in Arabidopsis
Plant Physiology, November 1, 2005; 139(3): 1291 - 1303.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. H. Foyer and G. Noctor
Redox Homeostasis and Antioxidant Signaling: A Metabolic Interface between Stress Perception and Physiological Responses
PLANT CELL, July 1, 2005; 17(7): 1866 - 1875.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
Z. Chen and D. R. Gallie
Increasing Tolerance to Ozone by Elevating Foliar Ascorbic Acid Confers Greater Protection against Ozone Than Increasing Avoidance
Plant Physiology, July 1, 2005; 138(3): 1673 - 1689.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.