Skip Navigation



JXB Advance Access published online on September 24, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh254
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/408/2483    most recent
erh254v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Mir, G.
Right arrow Articles by Molinas, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mir, G.
Right arrow Articles by Molinas, M.
Agricola
Right arrow Articles by Mir, G.
Right arrow Articles by Molinas, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received April 30, 2004
Accepted July 19, 2004

RESEARCH PAPER

A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress

Gisela Mir 1, Jordi Domènech 2, Gemma Huguet 1, Woei-Jiun Guo 3, Peter Goldsbrough 3, Silvia Atrian 2*, and Marisa Molinas 1

1 Departament de Biologia, Universitat de Girona, Campus Montilivi, E-17071 Girona, Spain
2 Departament de Genètica, Universitat de Barcelona, Av. Diagonal 645, E-08028 Barcelona, Spain
3 Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA

* To whom correspondence should be addressed. E-mail: satrian{at}ub.edu.


  Abstract

Expression of plant metallothionein genes has been reported in a variety of senescing tissues, such as leaves and stems, ripening fruits, and wounded tissues, and has been proposed to function in both metal chaperoning and scavenging of reactive oxygen species. In this work, it is shown that MT is also associated with suberization, after identifying a gene actively transcribed in Quercus suber cork cells as a novel MT. This cDNA, isolated from a phellem cDNA library, encodes a MT that belongs to type 2 plant MTs (QsMT). Expression of the QsMT cDNA in E. coli grown in media supplemented with Zn, Cd, or Cu has yielded recombinant QsMT. Characterization of the respective metal aggregates agrees well with a copper-related biological role, consistent with the capacity of QsMT to restore copper tolerance to a MT-deficient, copper-sensitive yeast mutant. Furthermore, in situ hybridization results demonstrate that RNA expression of QsMT is mainly observed under conditions related to oxidative stress, either endogenous, as found in cork or in actively proliferating tissues, or exogenous, for example, in response to H2O2 or paraquat treatments. The putative role of QsMT in oxidative stress, both as a free radical scavenger via its sulphydryl groups or as a copper chelator is discussed.

Keywords: In situ hybridization; metal co-ordination; metallothionein; oxidative stress; senescent plant tissue; Quercus suber.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
G. Ramesh, G. K. Podila, G. Gay, R. Marmeisse, and M. S. Reddy
Different Patterns of Regulation for the Copper and Cadmium Metallothioneins of the Ectomycorrhizal Fungus Hebeloma cylindrosporum
Appl. Envir. Microbiol., April 15, 2009; 75(8): 2266 - 2274.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
H. Zhang, C. Lian, and Z. Shen
Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa
Ann. Bot., April 1, 2009; 103(6): 923 - 930.
[Abstract] [Full Text] [PDF]

Home page
 Tree PhysiolHome page
H. Shin, H. Lee, K.-S. Woo, E.-W. Noh, Y.-B. Koo, and K.-J. Lee
Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine
Tree Physiol, March 1, 2009; 29(3): 411 - 421.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
V. H. Hassinen, M. Tuomainen, S. Peraniemi, H. Schat, S. O. Karenlampi, and A. I. Tervahauta
Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens
J. Exp. Bot., January 1, 2009; 60(1): 187 - 196.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Xue, X. Li, W. Zhu, C. Wu, G. Yang, and C. Zheng
Cotton metallothionein GhMT3a, a reactive oxygen species scavenger, increased tolerance against abiotic stress in transgenic tobacco and yeast
J. Exp. Bot., January 1, 2009; 60(1): 339 - 349.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. Steffens and M. Sauter
Epidermal Cell Death in Rice Is Confined to Cells with a Distinct Molecular Identity and Is Mediated by Ethylene and H2O2 through an Autoamplified Signal Pathway
PLANT CELL, January 1, 2009; 21(1): 184 - 196.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL, November 1, 2007; 19(11): 3669 - 3691.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
A. S. Gesteira, F. Micheli, N. Carels, A. C. Da Silva, K. P. Gramacho, I. Schuster, J. N. Macedo, G. A. G. Pereira, and J. C. M. Cascardo
Comparative Analysis of Expressed Genes from Cacao Meristems Infected by Moniliophthora perniciosa
Ann. Bot., July 1, 2007; 100(1): 129 - 140.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Zhigang, L. Cuijie, Z. Yuangang, D. Yejie, A. Wachter, R. Gromes, and T. Rausch
Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings
J. Exp. Bot., November 1, 2006; 57(14): 3575 - 3582.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
L. Tio, L. Villarreal, S. Atrian, and M. Capdevila
The Zn- and Cd-Clusters of Recombinant Mammalian MT1 and MT4 Metallothionein Domains Include Sulfide Ligands.
Experimental Biology and Medicine, October 1, 2006; 231(9): 1522 - 1527.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.