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JXB Advance Access published online on September 24, 2004
Journal of Experimental Botany, doi:10.1093/jxb/erh257
© 2004 by Oxford University Press
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Received April 15, 2004
Accepted July 12, 2004

RESEARCH PAPER

Increased sucrose level and altered nitrogen metabolism in Arabidopsis thaliana transgenic plants expressing antisense chloroplastic fructose-1,6-bisphosphatase

Mariam Sahrawy 1*, Concepción Ávila 2, Ana Chueca 1, Francisco M. Cánovas 2, and Julio López-Gorgé 1

1 Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Prof. Albareda 1, 18008-Granada, Spain
2 Department of Molecular Biology and Biochemistry. University of Málaga, Campus de Teatinos, s/n, 29071-Málaga, Spain

* To whom correspondence should be addressed. E-mail: sahrawy{at}eez.csic.es.


  Abstract

The pea chloroplastic fructose-1,6-bisphosphatase (FBPase) antisense construct reduced the endogenous level of expression of the corresponding Arabidopsis thaliana gene. The reduction of foliar FBPase activity in the transformants T2 and T3 generation ranged from 20% to 42%, and correlated with lower levels of FBPase protein. FBPase antisense plants displayed different phenotypes with a clear increase in leaf fresh weight. Measurements of photosynthesis revealed a higher carbon-assimilation rate. Decreased FBPase activity boosted the foliar carbohydrate contents, with a shift in the sucrose:starch ratio, which reached a maximum of 0.99 when the activity loss was 41%. Nitrate reductase activity decreased simultaneously with an increase in glutamine synthetase activity, which could be explained in terms of ammonium assimilation regulation by sugar content. These results suggest the role of FBPase as a key enzyme in CO2 assimilation, and also in co-ordinating carbon and nitrogen metabolism.

Keywords: Antisense; Arabidopsis; fructose-1,6-bisphosphatase; nitrogen; sucrose; transgenic.
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