Skip Navigation



JXB Advance Access published online on October 22, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri001
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
56/409/123    most recent
eri001v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Boucheron, E.
Right arrow Articles by Chriqui, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boucheron, E.
Right arrow Articles by Chriqui, D.
Agricola
Right arrow Articles by Boucheron, E.
Right arrow Articles by Chriqui, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received March 19, 2004
Accepted August 2, 2004

RESEARCH PAPER

Ectopic expression of Arabidopsis CYCD2 and CYCD3 in tobacco has distinct effects on the structural organization of the shoot apical meristem

E. Boucheron 1, J. H. S. Healy 2, C. Bajon 1, A. Sauvanet 1, J. Rembur 1, M. Noin 1, M. Sekine 3, C. Riou Khamlichi 2, J. A. H. Murray 2, H. Van Onckelen 4, and D. Chriqui 1*

1 Université Pierre et Marie Curie, site Ivry-le Raphaël, Laboratoire CEMV-EA3494, case 150, 4 place Jussieu, F-75252 Paris Cedex 05, France
2 Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
3 Graduate School of Biology Sciences, Nara Institute of Science and Technology, Takayam 8916-5, Ikoma, Nara 630-01, Japan
4 University of Antwerp, Department of Biology, Universiteitsplein1, B-2610 Wilrijk, Belgium

* To whom correspondence should be addressed.
D. Chriqui, E-mail: dominique.chriqui{at}snv.jussieu.fr


  Abstract

Transgenic tobacco lines expressing Arath-CYCD2 or Arath-CYCD3 genes under a cauliflower mosaic virus 35S promoter are modified in the timing of their development, but not in the phenotype of their vegetative organs. They display an increased rate of leaf initiation, which is shown to be associated with distinct changes in the structural organization of their shoot apical meristem (SAM). Constitutive expression of Arath-CYCD2 leads to a progressive modification of the SAM structural organization with predominant periclinal divisions in the L3 layer and to the loss of the classical cytophysiological zonation, the central zone being reduced to the central cells of the L1 and L2 layers. These changes reveal a particular sensitivity of the corpus cells (L3) to Arath-CYCD2 over-expression and suggest a role for CYCD2 in controlling the planes of cell division in these cells. The SAM structural modifications in the Arath-CYCD3 over-expressing lines are less drastic; only an increased cell number together with a reduced cell size, particularly in the L1 layer, characterizes the peripheral zones. This could be related to the shortening of the G1-phase duration that renders cell growth incomplete between successive mitoses. Cell proliferation continues beyond the SAM in the developing internodes and confers a delayed senescence to Arath-CYCD3 over-expressing juvenile tissues. In addition, the ploidy levels of mature stem tissues in both types of transgenic lines are unaffected, suggesting that the studied G1 to S cell-cycle genes have no effect on the extent of endoreduplication in tobacco stem tissues.

Keywords: D-type cyclins; morphogenesis; plant cell cycle; shoot meristem; tobacco.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
J.-W. Wang, R. Schwab, B. Czech, E. Mica, and D. Weigel
Dual Effects of miR156-Targeted SPL Genes and CYP78A5/KLUH on Plastochron Length and Organ Size in Arabidopsis thaliana
PLANT CELL, May 1, 2008; 20(5): 1231 - 1243.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. U. Andersen, S. Buechel, Z. Zhao, K. Ljung, O. Novak, W. Busch, C. Schuster, and J. U. Lohmann
Requirement of B2-Type Cyclin-Dependent Kinases for Meristem Integrity in Arabidopsis thaliana
PLANT CELL, January 1, 2008; 20(1): 88 - 100.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Menges, A. K. Samland, S. Planchais, and J. A.H. Murray
The D-Type Cyclin CYCD3;1 Is Limiting for the G1-to-S-Phase Transition in Arabidopsis
PLANT CELL, April 1, 2006; 18(4): 893 - 906.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.