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JXB Advance Access published online on November 1, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri007
© 2004 by Oxford University Press
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Received May 13, 2004
Accepted August 12, 2004

RESEARCH PAPER

Effect of sucrose on ascorbate level and expression of genes involved in the ascorbate biosynthesis and recycling pathway in harvested broccoli florets

Fumie Nishikawa 1*, Masaya Kato 2, Hiroshi Hyodo 3, Yoshinori Ikoma 2, Minoru Sugiura 2, and Masamichi Yano 2

1 The United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Yanagido, Gifu, 501-1193 Japan; Department of Biological Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka, 422-8529 Japan; Department of Citrus Research, National Institute of Fruit Tree Science, Shimizu-okitsunakacho, Shizuoka, 424-0292 Japan
2 Department of Citrus Research, National Institute of Fruit Tree Science, Shimizu-okitsunakacho, Shizuoka, 424-0292 Japan
3 Department of Biological Sciences, Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka, 422-8529 Japan

* To whom correspondence should be addressed.
Fumie Nishikawa, E-mail: fumien{at}affrc.go.jp


  Abstract

The relationship between sucrose (Suc) and ascorbate (AA) metabolism was investigated in harvested broccoli (Brassica oleracea L. var. italica) florets. Decreases in both Suc and AA content were observed in broccoli florets 48 h after all the leaves were excised, but none were observed when the plants were kept intact or with leaves attached in a room at 20 °C. In harvested broccoli plants without leaves and roots, continuous absorption of a 10% (w/v) Suc solution from the cut surface of the stem suppressed the degreening of sepals and the loss of AA content in florets. The expression of the genes related to AA metabolism in chloroplasts and its biosynthesis were up-regulated by Suc feeding in broccoli florets. These data suggest that a decline in Suc leads to considerable damage not only to AA biosynthesis but also to the hydrogen peroxide-scavenging system in chloroplasts. In addition, the cessation of the Suc supply from leaves can be the main factor of AA degradation in harvested broccoli florets.

Keywords: Ascorbate; Brassica oleracea; gene expression; harvest; sucrose.
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