Skip Navigation



JXB Advance Access published online on November 8, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri016
© 2004 by Oxford University Press
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
56/409/81    most recent
eri016v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ducreux, L. J. M.
Right arrow Articles by Taylor, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ducreux, L. J. M.
Right arrow Articles by Taylor, M. A.
Agricola
Right arrow Articles by Ducreux, L. J. M.
Right arrow Articles by Taylor, M. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received May 21, 2004
Accepted August 24, 2004

RESEARCH PAPER

Metabolic engineering of high carotenoid potato tubers containing enhanced levels of {beta}-carotene and lutein

Laurence J. M. Ducreux 1, Wayne L. Morris 1, Peter E. Hedley 2, Tom Shepherd 1, Howard V. Davies 1, Steve Millam 2, and Mark A. Taylor 1*

1 Quality, Health and Nutrition, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK
2 Gene Expression, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

* To whom correspondence should be addressed.
Mark A. Taylor, E-mail: mtaylo{at}scri.sari.ac.uk


  Abstract

In order to enhance the carotenoid content of potato tubers, transgenic potato plants have been produced expressing an Erwinia uredovora crtB gene encoding phytoene synthase, specifically in the tuber of Solanum tuberosum L. cultivar Désirée which normally produces tubers containing c. 5.6 µg carotenoid g-1 DW and also in Solanum phureja L. cv. Mayan Gold which has a tuber carotenoid content of typically 20 µg carotenoid g-1 DW. In developing tubers of transgenic crtB Désirée lines, carotenoid levels reached 35 µg carotenoid g-1 DW and the balance of carotenoids changed radically compared with controls: {beta}-carotene levels in the transgenic tubers reached c. 11 µg g-1 DW, whereas control tubers contained negligible amounts and lutein accumulated to a level 19-fold higher than empty-vector transformed controls. The crtB gene was also transformed into S. phureja (cv. Mayan Gold), again resulting in an increase in total carotenoid content to 78 µg carotenoid g-1 DW in the most affected transgenic line. In these tubers, the major carotenoids were violaxanthin, lutein, antheraxanthin, and {beta}-carotene. No increases in expression levels of the major carotenoid biosynthetic genes could be detected in the transgenic tubers, despite the large increase in carotenoid accumulation. Microarray analysis was used to identify a number of genes that were consistently up- or down-regulated in transgenic crtB tubers compared with empty vector controls. The implications of these data from a nutritional standpoint and for further modifications of tuber carotenoid content are discussed.

Keywords: {beta}-Carotene; carotenoids; lutein; microarray; potato; transgenic.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
M. Fujisawa, E. Takita, H. Harada, N. Sakurai, H. Suzuki, K. Ohyama, D. Shibata, and N. Misawa
Pathway engineering of Brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation
J. Exp. Bot., March 1, 2009; 60(4): 1319 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Zhu, S. Naqvi, J. Breitenbach, G. Sandmann, P. Christou, and T. Capell
Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize
PNAS, November 25, 2008; 105(47): 18232 - 18237.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Aluru, Y. Xu, R. Guo, Z. Wang, S. Li, W. White, K. Wang, and S. Rodermel
Generation of transgenic maize with enhanced provitamin A content
J. Exp. Bot., October 1, 2008; 59(13): 3551 - 3562.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Newell-McGloughlin
Nutritionally Improved Agricultural Crops
Plant Physiology, July 1, 2008; 147(3): 939 - 953.
[Full Text] [PDF]

Home page
 J Exp BotHome page
A. B. Lopez, J. Van Eck, B. J. Conlin, D. J. Paolillo, J. O'Neill, and L. Li
Effect of the cauliflower Or transgene on carotenoid accumulation and chromoplast formation in transgenic potato tubers
J. Exp. Bot., February 5, 2008; (2008) erm299v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Lu, J. Van Eck, X. Zhou, A. B. Lopez, D. M. O'Halloran, K. M. Cosman, B. J. Conlin, D. J. Paolillo, D. F. Garvin, J. Vrebalov, et al.
The Cauliflower Or Gene Encodes a DnaJ Cysteine-Rich Domain-Containing Protein That Mediates High Levels of {beta}-Carotene Accumulation
PLANT CELL, December 1, 2006; 18(12): 3594 - 3605.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
T. Gerjets and G. Sandmann
Ketocarotenoid formation in transgenic potato
J. Exp. Bot., November 1, 2006; 57(14): 3639 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. L. Morris, L. J. M. Ducreux, P. Hedden, S. Millam, and M. A. Taylor
Overexpression of a bacterial 1-deoxy-D-xylulose 5-phosphate synthase gene in potato tubers perturbs the isoprenoid metabolic network: implications for the control of the tuber life cycle
J. Exp. Bot., September 1, 2006; 57(12): 3007 - 3018.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Al-Babili, T. T. C. Hoa, and P. Schaub
Exploring the potential of the bacterial carotene desaturase CrtI to increase the {beta}-carotene content in Golden Rice
J. Exp. Bot., March 1, 2006; 57(4): 1007 - 1014.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.