JXB Advance Access published online on November 8, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri017
© 2004 by Oxford University Press
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1 Department of Biochemistry and Physiology of Plants W5, University of Bielefeld, University Str. 25, D-33501, Bielefeld, Germany; Department of Botany, Faculty of Science, University of Assiut, 71516, Assiut, Egypt
* To whom correspondence should be addressed. This work evaluates the (cor-)relations between selected biochemical responses to toxic Cd and the degree of Cd sensitivity in a set of pea genotypes. Ten genotypes were analysed that differ in their growth response to Cd when expressed as root or shoot tolerance indices (TIs). Concentrations of non-protein thiols (NPTs) and malondialdehyde (MDA), activity of chitinase, peroxidase (POX), and catalase significantly increased in all pea genotypes treated with Cd. Cd-sensitivity of genotypes was correlated with relative increases in MDA concentration as well as activities of chitinase and POX, suggesting similar Cd stress effects. Activities of ascorbate peroxidase (APX) decreased, but concentrations of glutathione (GSH) increased in the less Cd-sensitive genotypes. Differences in root and leaf contents of Cd revealed no correlation with TI, metabolic parameters, and enzyme activities in Cd-treated plants, respectively, except that shoot Cd concentration positively correlated with shoot chitinase activity. Toxic Cd levels inhibited uptake of nutrient elements such as P, K, S, Ca, Zn, Mn, and B by plants in an organ- and genotype-specific manner. Cd-sensitivity was significantly correlated with decreased root Zn concentrations. The results show both similarities, as well as distinct features, in Cd toxicity expression in genotypes of one species, suggesting that independent and multi-factorial reactions modulate Cd sensitivity on the low-tolerance level of plants. The study illustrates the biochemical basis of earlier detected genotypic variation in Cd response.
Accepted August 25, 2004
RESEARCH PAPER
Genotypic variation of the response to cadmium toxicity in Pisum sativum L
2 All-Russia Research Institute for Agricultural Microbiology, Podbelskogo 3, Pushkin 8, 196608, St Petersburg, Russian Federation
3 Department of Biochemistry and Physiology of Plants W5, University of Bielefeld, University Str. 25, D-33501, Bielefeld, Germany
Karl-Josef Dietz, E-mail: karl-josef.dietz{at}uni-bielefeld.de
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