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JXB Advance Access published online on November 22, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri028
© 2004 by Oxford University Press
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Received May 26, 2004
Accepted September 13, 2004

RESEARCH PAPER

Reduction of cell size induced by enod40 in Arabidopsis thaliana

Flavia Guzzo 1, Paolo Portaluppi 1, Romeo Grisi 1, Silvia Barone 1, Stefania Zampieri 1, Henk Franssen 2, and Marisa Levi 1*

1 University of Verona, Dipartimento Scientifico e Tecnologico, Strada le Grazie 15, Cà Vignal 1, 37134 Verona, Italy
2 Wageningen University, Department of Molecular Biology, Drijenlaan 3, 6703 HA Wageningen, The Netherlands

* To whom correspondence should be addressed.
Marisa Levi, E-mail: marisa.levi{at}univr.it


  Abstract

An extensive analysis of organ and cell size was performed in three different Arabidopsis lines transformed with the early nodulin gene enod40 under control of the CaMV35S promoter. All three transgenic lines presented a significant decrease in the mean size of both epidermal internode and leaf mesophyll cells. Flow cytometric and image analysis of enod40-transfected protoplasts prepared from wild-type Arabidopsis cell suspensions showed that transient expression of the gene resulted in reduced forward light scattering (a factor correlated with particle size) and cell size. The direct administration of ENOD40 peptide to fresh protoplasts also resulted in reduced forward scattering with respect to the control and to the administration of unrelated peptides. As far as is known this is the first report documenting a biological effect of enod40 at the cellular level in non-legume plants.

Keywords: Arabidopsis; cell size; enod40; flow cytometry; GFP.
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