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JXB Advance Access published online on December 6, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri035
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Journal of Experimental Botany © Society for Experimental Biology 2004; all rights reserved
Received September 9, 2004
Accepted September 22, 2004

RESEARCH PAPER

Tight regulation of expression of two Arabidopsis cytosolic Hsp90 genes during embryo development

Constantinos Prasinos 1, Konstantinos Krampis 2, Despina Samakovli 3, and Polydefkis Hatzopoulos 3*

1 Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece; Present address: Department of Forestry, Michigan State University, East Lansing, MI 48824-1222, USA
2 Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece; Present address: Virginia Bioinformatics Institute, Washington Street, 0477 Virginia Tech, Blacksburg, VA 24061, USA
3 Laboratory of Molecular Biology, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55 Athens, Greece

* To whom correspondence should be addressed.
Polydefkis Hatzopoulos, E-mail: phat{at}aua.gr


  Abstract

The spatial and temporal distribution of expression of two cytosolic members of the AtHsp90 gene family was assessed during early development. In stressed transgenic plants bearing the AtHsp90-3 promoter, {beta}-glucuronidase (GUS) activity was strong in meristematic tissues. Expression was also detected in vascular tissues, leaf veins, siliques, and in pollen sacs. The promoter induced gene expression after heat shock in a time-course dependent manner. AtHsp90-1 promoter activity was low throughout the early stages of embryo development but high just before embryo maturation, with expression most prominent in cotyledons. AtHsp90-3 promoter activity was almost constant and restricted to the root and the cotyledon tips of the embryo. This highly specific spatial distribution of GUS activity changed when the tissues were heat-stressed. Both promoters were also active in unstressed mature pollen grains and during pollen germination. The results shown here indicate that different regulatory and developmental mechanisms control and differentiate the expression of the two cytosolic members of the Arabidopsis AtHsp90 gene family under normal conditions. The developmental and restricted pattern of expression of the AtHsp90-1 and -3 gene promoters in unstressed transgenic plants suggest prominent and distinctive roles of these two genes during different developmental processes.

Keywords: Arabidopsis; Hsp90 genes; pollen and embryo expression; promoter analysis.
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D. Samakovli, A. Thanou, C. Valmas, and P. Hatzopoulos
Hsp90 canalizes developmental perturbation
J. Exp. Bot., October 1, 2007; 58(13): 3513 - 3524.
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