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JXB Advance Access published online on December 23, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri038
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Journal of Experimental Botany © Society for Experimental Biology 2004; all rights reserved
Received June 9, 2004
Accepted September 23, 2004

RESEARCH PAPER

Stress-responsive {alpha}-dioxygenase expression in tomato roots

Ananchanok Tirajoh 1, Theingi S. T. Aung 1, Ashley Byun McKay 1, and Aine L. Plant 1*

1 Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC, Canada V5A 1S6

* To whom correspondence should be addressed.
Aine L. Plant, E-mail: aplant{at}sfu.ca


  Abstract

Alpha-dioxygenase ({alpha}-DOX) enzymes catalyse the oxygenation of fatty acids to yield a newly identified group of oxylipins that play a role in protecting tissues from oxidative damage and cell death. In tomato (Lycopersicon esculentum Mill.) {alpha}-DOX was identified as salt-regulated by differential display of mRNA, and is represented by a small gene family comprising at least three members: LE{alpha}-DOX1, -2, and -3 of which only LE{alpha}-DOX1 was salt-responsive. The enhancement of LE{alpha}-DOX1 expression in roots by salt, wounding and challenge with Pythium aphanidermatum (Edson) Fitzp. suggests that {alpha}-DOX-generated oxylipins may mediate the response of roots to these environmental stresses. In roots, LE{alpha}-DOX1 was abscisic acid (ABA)-responsive. However, in the ABA-deficient mutant flacca salt-responsive expression was equivalent to that in the wild type. Similarly, in roots exposed to fluridone (FLU) salt up-regulated expression; however, in this case salt-responsive LE{alpha}-DOX1 expression was greater than that in roots that were not exposed to FLU. A possible explanation for this is provided by the role of ABA in suppressing ethylene accumulation in osmotically stressed roots. The ethylene-generating agent ethephon and precursor 1-aminocyclopropane-1-carboxylic acid markedly elevated LE{alpha}-DOX1 expression, and this enhanced expression was suppressed by ABA. LE{alpha}-DOX1 expression in salt-stressed roots was not markedly affected by AVG indicating that ABA may be responsible for enhanced {alpha}-DOX expression in salt-treated roots.

Keywords: Abscisic acid; {alpha}-dioxygenase; ethylene; Lycopersicon esculentum; oxylipin; roots; salt stress.
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