JXB Advance Access published online on January 10, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri050
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1 Institut des Sciences du Végétal, UPR 2355 CNRS, 1. avenue de la terrasse, 91198 Gif-sur-Yvette cedex, France; Present address and to whom correspondence should be sent: John Innes Centre, Cell and Developmental Biology Department, Colney Lane, Norwich NR4 7UH, UK. Fax: +44 (0)1603 450 025
* To whom correspondence should be addressed. The Arabidopsis thaliana genome contains approximately 80 genes encoding basic leucine zipper transcription factors, divided into 11 groups. Abscisic Acid-Insensitive 5 (ABI5) is one of the 13 members of group A and is involved in ABA signalling during seed maturation, and germination. Seven other members of this group are expressed during seed maturation, but only one of them (Enhanced Em Level, EEL) has been functionally characterized during this developmental phase. Since EEL and two other group A genes, AtbZIP67 and AREB3 (ABA-Responsive Element Binding protein 3), display similar mRNA temporal expression in whole siliques, it is suspected that they might share some overlapping functions. To address this question, the proteins' tissular and subcellular localization in transgenic Arabidopsis were precisely characterized, using translational fusions with a green fluorescent protein (GFP) expressed under the corresponding bZIP promoter. It was found that the three fusion proteins were expressed with a largely overlapping pattern and constitutively localized in the nuclei. An RNA interference approach (RNAi) was then used to knock out the expression of all three genes simultaneously. Endogenous EEL, AREB3, and AtbZIP67 transcripts could be specifically reduced, but no visible defects could be observed during seed maturation.
Received June 24, 2004
Accepted October 4, 2004
RESEARCH PAPER
Characterization of three homologous basic leucine zipper transcription factors (bZIP) of the ABI5 family during Arabidopsis thaliana embryo maturation
2 Institut des Sciences du Végétal, UPR 2355 CNRS, 1. avenue de la terrasse, 91198 Gif-sur-Yvette cedex, France
3 Institut des Sciences du Végétal, UPR 2355 CNRS, 1. avenue de la terrasse, 91198 Gif-sur-Yvette cedex, France; Present address: Laboratoire DRDC/PCV, UMR CEA-CNRS 5168-INRA 1200-UJF, CEA, 17 rue des Martyrs, bât. C2, 38054 Grenoble Cedex 9, France
Sandra Bensmihen, E-mail: sandra.bensmihen{at}bbsrc.ac.uk
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