Skip Navigation



JXB Advance Access published online on December 13, 2004
Journal of Experimental Botany, doi:10.1093/jxb/eri059
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary tables
Right arrow All Versions of this Article:
56/410/309    most recent
eri059v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Urbanczyk-Wochniak, E.
Right arrow Articles by Fernie, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Urbanczyk-Wochniak, E.
Right arrow Articles by Fernie, A. R.
Agricola
Right arrow Articles by Urbanczyk-Wochniak, E.
Right arrow Articles by Fernie, A. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany © Society for Experimental Biology 2004; all rights reserved
Received June 25, 2004
Accepted October 22, 2004

Metabolomics and Metabolic Profiling Special Issue Article

Metabolic profiling reveals altered nitrogen nutrient regimes have diverse effects on the metabolism of hydroponically-grown tomato (Solanum lycopersicum) plants

Ewa Urbanczyk-Wochniak 1 and Alisdair R. Fernie 1*

1 Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Golm, Germany

* To whom correspondence should be addressed.
Alisdair R. Fernie, E-mail: fernie{at}mpimp-golm.mpg.de


  Abstract

The role of inorganic nitrogen assimilation in the production of amino acids is one of the most important biochemical processes in plants. For this reason, a detailed broad-range characterization of the metabolic response of tomato (Solanum lycopersicum) leaves to the alteration of nitrate level was performed. Tomato plants were grown hydroponically in liquid culture under three different nitrate regimes: saturated (8 mM NO3-), replete (4 mM NO3-) and deficient (0.4 mM NO3-). All treatments were performed under varied light intensity, with leaf samples being collected after 7, 14, and 21 d. In addition, the short-term response (after 1, 24, 48, and 94 h) to varying nutrient status was evaluated at the higher light intensity. GC-MS analysis of the levels of amino acids, tricarboxylic acid cycle intermediates, sugars, sugar alcohols, and representative compounds of secondary metabolism revealed substantial changes under the various growth regimes applied. The data presented here suggest that nitrate nutrition has wide-ranging effects on plant leaf metabolism with nitrate deficiency resulting in decreases in many amino and organic acids and increases in the level of several carbohydrates and phosphoesters, as well as a handful of secondary metabolites. These results are compared with previously reported transcript profiles of altered nitrogen regimes and discussed within the context of current models of carbon nitrogen interaction.

Keywords: Amino acids; carbon metabolism; light intensity; nitrate reduction; nitrogen metabolism; organic acids; Solanum lycopersicum; tomato.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. L. Bot, C. Benard, C. Robin, F. Bourgaud, and S. Adamowicz
The 'trade-off' between synthesis of primary and secondary compounds in young tomato leaves is altered by nitrate nutrition: experimental evidence and model consistency
J. Exp. Bot., November 1, 2009; 60(15): 4301 - 4314.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
R. Huanosto Magana, S. Adamowicz, and L. Pages
Diel changes in nitrogen and carbon resource status and use for growth in young plants of tomato (Solanum lycopersicum)
Ann. Bot., May 1, 2009; 103(7): 1025 - 1037.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Jumtee, T. Bamba, A. Okazawa, E. Fukusaki, and A. Kobayashi
Integrated metabolite and gene expression profiling revealing phytochrome A regulation of polyamine biosynthesis of Arabidopsis thaliana
J. Exp. Bot., April 1, 2008; 59(6): 1187 - 1200.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. G. Forde and P. J. Lea
Glutamate in plants: metabolism, regulation, and signalling
J. Exp. Bot., July 1, 2007; 58(9): 2339 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. S. Sharma and K.-J. Dietz
The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress
J. Exp. Bot., March 1, 2006; 57(4): 711 - 726.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.