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JXB Advance Access published online on January 10, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri066
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Journal of Experimental Botany © Society for Experimental Biology 2005; all rights reserved
Received October 25, 2004
Accepted October 28, 2004

REVIEW ARTICLE

Microgenomics: gene expression analysis at the tissue-specific and single-cell levels

Stephan P. Brandt 1*

1 Technische Universität Darmstadt, Institut für Botanik, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany

* To whom correspondence should be addressed.
Stephan P. Brandt, E-mail: sbrandt{at}bio.tu-darmstadt.de


  Abstract

Plant organs are composed of many different cell types and the analysis of ‘bulk’ material results in the average of all information in these cells. Therefore, this does not reflect any individuality of the tissues present in plants. This review briefly summarizes different sampling methods which provide tissue- and cell-specific samples, respectively. In addition, gene expression analysis tools that allow the analysis of transcripts in minute samples are discussed in detail. The combination of both approaches results in high resolution gene expression data, which increases understanding of plant physiology in such diverse areas as primary and secondary metabolism, plant defence or stress response.

Keywords: Array hybridization; cDNA libraries; gene expression pattern; glass capillary; homomeric tailing; linear antisense amplification; microdissection; RT-PCR; single cell analysis; tissue specificity.
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