JXB Advance Access published online on February 14, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri095
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Peking University, College of Life Sciences Beijing 100871, PR China; Commissariat Général à l'Energie Atomique, Centre Régional d'Etudes Nucléaires de Kinshasa (CGEA/CREN-K), B.P. 868, Kinshasa XI, Rép. Dém. du Congo
* To whom correspondence should be addressed. The seasonal change of free abscisic acid (ABA) and indole-3-acetic acid (IAA) and their relationship with the cambial activity in Eucommia ulmoides trees were investigated by ABA and IAA immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, ABA and IAA quantification using high performance liquid chromatography (HPLC), and systematic monitoring of vascular cell layers production. ABA and IAA clearly displayed opposite annual distribution patterns. In the active period (AP), both immunolocalization and HPLC detected an abrupt decrease of ABA, reaching its lowest level in the summer. During dormancy, ABA started increasing in the first quiescence (Q1) (autumn), peaked in the rest (winter), and gradually decreased from the onset of the second quiescence (Q2) (the end of winter). IAA showed a reverse pattern to that of ABA: it sharply increased in AP, but noticeably decreased from the commencement of Q1. Longitudinally, the ABA distribution increased apico-basally, contrasting with IAA. Laterally, most of the ABA was located in mature vascular tissues, whereas the IAA essentially occurred in the cambial region. The concomitant IAA-ABA distribution and seasonal changes in vascular tissues greatly correlated with xylem and phloem cell production, and late wood differentiation and maturation. Interestingly, the application of exogenous ABA to quiescent E. ulmoides branches, in a water-culture system, inhibited external IAA action on cambial activity reactivation. These results suggest that, in E. ulmoides, ABA and IAA might probably interact in the cambial region. The annual cambial activity could be influenced by an IAA:ABA ratio; and ABA might play a key role in vascular cambium dormancy in higher plants. The relationship between hormonal changes and the (particular) annual life cycle of E. ulmoides is also discussed. *These authors contributed equally to this study.
Received November 23, 2004
Accepted December 3, 2004
RESEARCH PAPER
Opposite patterns in the annual distribution and time-course of endogenous abscisic acid and indole-3-acetic acid in relation to the periodicity of cambial activity in Eucommia ulmoides Oliv
2 Peking University, College of Life Sciences Beijing 100871, PR China
3 China Agricultural University, College of Biology, Beijing 100094, PR China
Ke-Ming Cui, E-mail: ckm{at}pku.edu.cn
Abstract
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Volaire, G. Seddaiu, L. Ledda, and F. Lelievre Water deficit and induction of summer dormancy in perennial Mediterranean grasses Ann. Bot., June 1, 2009; 103(8): 1337 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kong, S. R. Abrams, S. J. Owen, A. Van Niejenhuis, and P. Von Aderkas Dynamic changes in concentrations of auxin, cytokinin, ABA and selected metabolites in multiple genotypes of Douglas-fir (Pseudotsuga menziesii) during a growing season Tree Physiol, February 1, 2009; 29(2): 183 - 190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Pang, J. Zhang, J. Cao, S.-Y. Yin, X.-Q. He, and K.-M. Cui Phloem transdifferentiation from immature xylem cells during bark regeneration after girdling in Eucommia ulmoides Oliv J. Exp. Bot., April 1, 2008; 59(6): 1341 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-W. Hou, Y.-T. Zhou, K.-N. Mwange, W.-F. Li, X.-Q. He, and K.-M. Cui ABP1 expression regulated by IAA and ABA is associated with the cambium periodicity in Eucommia ulmoides Oliv. J. Exp. Bot., November 1, 2006; 57(14): 3857 - 3867. [Abstract] [Full Text] [PDF]
|
|