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JXB Advance Access published online on February 28, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri105
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Journal of Experimental Botany © Society for Experimental Biology 2005; all rights reserved
Received August 17, 2004
Accepted December 16, 2004

RESEARCH PAPER

Characterization and functional investigation of an Arabidopsis cDNA encoding a homologue to the d-PGMase superfamily

Fabienne Bourgis 1, Fredrik C. Botha 2, Srikrishnan Mani 3, Fletcher N. Hiten 4, Daniel J. Rigden 5, and Nathalie Verbruggen 6*

1 Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, CP 242, Bd du Triomphe, B-1050 Bruxelles, Belgium; Vakgroep Moleculaire Genetica and Department of Plant Systems Biology, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Technologiepark 927, B-9052 Gent, Belgium; Present address: Institut de Recherche pour le Développement, 911 Avenue Agropolis, BP 64501; F-34394 Montpellier cedex 5, France
2 Institute for Plant Biotechnology, University of Stellenbosch, Private Bag, 7602 Matieland, South Africa; Present address: South African Sugarcane Research Institute, Private Bag X02, Mount Edgecombe, 4300, South Africa
3 Vakgroep Moleculaire Genetica and Department of Plant Systems Biology, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Technologiepark 927, B-9052 Gent, Belgium; Present Address: Innogenetics, Technologiepark 7, B-9052 Gent, Belgium
4 Institute for Plant Biotechnology, University of Stellenbosch, Private Bag, 7602 Matieland, South Africa
5 National Centre of Genetic Resources and Biotechnology, Cenargen/Embrapa, S.A.I.N. Parque Rural, Final W3, Asa Norte, 70770-900 Brasilia, Brazil
6 Laboratoire de Physiologie et de Génétique Moléculaire des Plantes, Université Libre de Bruxelles, CP 242, Bd du Triomphe, B-1050 Bruxelles, Belgium; Vakgroep Moleculaire Genetica and Department of Plant Systems Biology, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Technologiepark 927, B-9052 Gent, Belgium

* To whom correspondence should be addressed.
Nathalie Verbruggen, E-mail: nverbru{at}ulb.ac.be


  Abstract

An Arabidopsis thaliana cDNA (At-74) has been isolated that encoded an uncharacterized protein showing homology with members of the d-PGMase superfamily: cofactor-dependent phosphoglycerate mutases (d-PGMases) and the phosphatase domain of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases (6PF2Kase/F2, 6Pase). Preliminary phylogenetic studies indicated that At-74 cDNA and its close homologue in Arabidopsis, At-74H, belong, however, to an equally distinct group. At-74 was ubiquitously expressed in vegetative organs and induced by glucose. The At-74 cDNA was overexpressed in A. thaliana to investigate its function, but this overexpression did not result in a clear phenotype. Enzymatic assays performed on At-74-overproducing transgenic plants or E. coli cells showed no increase in either the activities of cofactor-dependent and -independent phosphoglycerate mutases (i-PGMases) and F2,6Pase or that of acid phosphatases. The possible role of At-74 in plant metabolism was further investigated by carbon partitioning experiments with [U-14C] glucose and measurements of soluble sugars in both young leaves and roots. Two overexpressing At-74 lines showed a clear increase in glucose uptake. This paper introduces the At-74 homologue of the d-PGMase superfamily members and supports a possible role of At-74 in carbohydrate metabolism.

Keywords: d-PGMase family; transgenic plants; [U-14C]glucose feeding.
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