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JXB Advance Access published online on February 21, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri109
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Journal of Experimental Botany © Society for Experimental Biology 2005; all rights reserved
Received August 13, 2004
Accepted December 20, 2004

RESEARCH PAPER

Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petuniaxhybrida and Dendranthemaxgrandiflorum

Mariya Khodakovskaya 1, Yi Li 2, Jisheng Li 2, Radomíra Vanková 3, Jirí Malbeck 3, and Richard McAvoy 2*

1 University of Connecticut, Plant Science Department, Agricultural Biotechnology Laboratories, 1390 Storrs Rd, U-4163, Storrs, CT 06269-4163, USA; Institute Biology and Soil Sciences, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok, Russian Federation
2 University of Connecticut, Plant Science Department, Agricultural Biotechnology Laboratories, 1390 Storrs Rd, U-4163, Storrs, CT 06269-4163, USA
3 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic

* To whom correspondence should be addressed.
Richard McAvoy, E-mail: Richard.McAvoy{at}uconn.edu


  Abstract

To prevent leaf senescence of young transplants or excised shoots during storage under dark and cold conditions, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of a cold-inducible promoter cor15a from Arabidopsis thaliana and introduced into Petuniaxhybrida ‘Marco Polo Odyssey’ and Dendranthemaxgrandiflorum (chrysanthemum) ‘Iridon’. Transgenic cor15a-ipt petunia and chrysanthemum plants and excised leaves remained green and healthy during prolonged dark storage (4 weeks at 25 °C) after an initial exposure to a brief cold-induction period (4 °C for 72 h). However, cor15a-ipt chrysanthemum plants and excised leaves that were not exposed to a cold-induction period, senesced under the same dark storage conditions. Regardless of cold-induction treatment, leaves and plants of non-transformed plants senesced under prolonged dark storage. Analysis of ipt expression indicated a marked increase in gene expression in intact transgenic plants as well as in isolated transgenic leaves exposed to a short cold-induction treatment prior to dark storage. These changes correlated with elevated concentrations of cytokinins in transgenic leaves after cold treatment. Cor15a-ipt transgenic plants showed a normal phenotype when grown at 25 °C.

Keywords: Cold-inducible promoter; cytokinins; ipt expression; leaf senescence; ornamental plants.
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