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JXB Advance Access published online on April 4, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri142
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received December 21, 2004
Accepted February 22, 2005

RESEARCH PAPER

VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem

Céline Vignault 1, Magali Vachaud 1, Birsen Cakir 1, David Glissant 1, Fabienne Dédaldéchamp 1, Michael Büttner 2, Rossitza Atanassova 1, Pierrette Fleurat-Lessard 1, Rémi Lemoine 1, and Serge Delrot 1*

1 Unité Mixte de Recherches Centre National de la Recherche Scientifique 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaire Végétales, Bâtiment Botanique, Université de Poitiers, 40 Avenue du Recteur Pineau, F-86022 Poitiers Cédex, France
2 Molekulare Pflanzenphysiologie, Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058, Germany

* To whom correspondence should be addressed.
Serge Delrot, E-mail: serge.delrot{at}univ-poitiers.fr


  Abstract

The accumulation of sugars in grape berries requires the co-ordinate expression of sucrose transporters, invertases, and monosaccharide transporters. A monosaccharide transporter homologue (VvHT1, Vitis vinifera hexose transporter 1) has previously been isolated from grape berries at the véraison stage, and its expression was shown to be regulated by sugars and abscisic acid. The present work investigates the function and localization of VvHT1. Heterologous expression in yeast indicates that VvHT1 encodes a monosaccharide transporter with maximal activity at acidic pH (pH 4.5) and high affinity for glucose (K m=70 µM). Fructose, mannose, sorbitol, and mannitol are not transported by VvHT1. In situ hybridization shows that VvHT1 transcripts are primarily found in the phloem region of the conducting bundles. Immunofluorescence and immunogold labelling experiments localized VvHT1 in the plasma membrane of the sieve element/companion cell interface and of the flesh cells. The expression and functional properties of VvHT1 suggests that it retrieves the monosaccharides needed to provide the energy necessary for cell division and cell growth at an early stage of berry development.

Keywords: Assimilate transport; grapevine; monosaccharide transport; ripening; unloading.
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