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JXB Advance Access published online on May 16, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri168
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
Received November 1, 2004
Accepted March 21, 2005

RESEARCH PAPER

Functional characterization of the thi1 promoter region from Arabidopsis thaliana

Denise Teixeira Ribeiro 1, Leonardo Paiva Farias 1, Juliana Dantas de Almeida 2, Priscila Mayumi Kashiwabara 1, Alberto F. C. Ribeiro 3, Marcio C. Silva-Filho 2, Carlos Frederico Martins Menck 4, and Marie-Anne Van Sluys 3*

1 Depto. de Botânica, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, 05508-900 São Paulo, SP, Brazil
2 Depto. de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Av. Pádua Dias, 11, 13400-970 Piracicaba, SP, Brazil
3 Depto. de Biologia, Instituto de Biociências, Universidade de São Paulo, Rua do Matão, 277, 05508-900 São Paulo, SP, Brazil
4 Depto. de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374, 05508-900 São Paulo, SP, Brazil

* To whom correspondence should be addressed.
Marie-Anne Van Sluys, E-mail: mavsluys{at}ib.usp.br


  Abstract

The Arabidopsis thaliana THI1 protein is involved in thiamine biosynthesis and is targeted to both chloroplasts and mitochondria by N-terminal control regions. To investigate thi1 expression, a series of thi1 promoter deletions were fused to the {beta}-glucuronidase (GUS) reporter gene. Transgenic plants were generated and expression patterns obtained under different environmental conditions. The results show that expression derived from the thi1 promoter is detected early on during development and continues throughout the plant's life cycle. High levels of GUS expression are observed in both shoots and roots during vegetative growth although, in roots, expression is restricted to the vascular system. Deletion analysis of the thi1 promoter region identified a region that is responsive to light. The smallest fragment (designated Pthi322) encompasses 306 bp and possesses all the essential signals for tissue specificity, as well as responsiveness to stress conditions such as sugar deprivation, high salinity, and hypoxia.

Keywords: GUS expression; promoter analysis; protein targeting; sugar modulation; thi1; thiamine biosynthesis; tissue expression pattern.
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