Skip Navigation



JXB Advance Access published online on July 12, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri227
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary material
Right arrow All Versions of this Article:
56/419/2345    most recent
eri227v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Sawant, S. V.
Right arrow Articles by Tuli, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sawant, S. V.
Right arrow Articles by Tuli, R.
Agricola
Right arrow Articles by Sawant, S. V.
Right arrow Articles by Tuli, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received February 14, 2005
Accepted May 16, 2005

RESEARCH PAPER

A variety of synergistic and antagonistic interactions mediated by cis-acting DNA motifs regulate gene expression in plant cells and modulate stability of the transcription complex formed on a basal promoter

Samir V. Sawant 1, Kanti Kiran 1, Rajesh Mehrotra 1, Chandra Prakash Chaturvedi 1, Suraiya A. Ansari 1, Pratibha Singh 1, Niraj Lodhi 1, and Rakesh Tuli 1*

1 National Botanical Research Institute, Rana Pratap Marg, Lucknow-226001, India

* To whom correspondence should be addressed.
Rakesh Tuli, E-mail: rakeshtuli{at}hotmail.com; rakeshtuli23@rediffmail.com


  Abstract

Several synthetic promoters containing a variety of commonly found cis-acting DNA sequence motifs were constructed to study the motif-motif and motif-protein interactions involved in gene expression in plants. Transient expression of the reporter gene gusA in tobacco leaves was used to demonstrate that several sequence elements can be arranged upstream of a basal promoter to function synergistically in enhancing gene expression. A cis-acting DNA motif could function as an activator by itself as well as a synergizing activator in the presence of other homologous as well as heterologous motifs in the neighbourhood. The function of a complex promoter comprising several activation motifs was arrested nearly completely in vivo, following titration with any one of the motifs. The results suggested a hierarchical assembly of several motif-binding factors, leading to the stabilization of the transcriptional complex formed on the TATA-box.

Keywords: Cis motif interactions; gene regulation; plant promoters; transcription activation in plants; transcription factors.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.