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JXB Advance Access published online on July 25, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri236
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
Received February 24, 2005
Accepted May 26, 2005

RESEARCH PAPER

GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana

Laurent Laplaze 1*, Boris Parizot 2, Andrew Baker 3, Lilian Ricaud 2, Alexandre Martinière 4, Florence Auguy 4, Claudine Franche 4, Laurent Nussaume 2, Didier Bogusz 4, and Jim Haseloff 3

1 UMR 1098, Institut de Recherche pour le Développement, 911 Avenue Agropolis, F-34394 Montpellier cedex 5, France; Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
2 Commissariat à l'Energie Atomique Cadarache, DSV, DEVM, Laboratoire de Biologie du Développement des Plantes, 6191 CNRS-CEA, Aix-Marseille II, F-13108 St Paul-lez-Durance Cedex, France
3 Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK
4 UMR 1098, Institut de Recherche pour le Développement, 911 Avenue Agropolis, F-34394 Montpellier cedex 5, France

* To whom correspondence should be addressed.
Laurent Laplaze, E-mail: laplaze{at}mpl.ird.fr


  Abstract

Lateral root development occurs throughout the life of the plant and is responsible for the plasticity of the root system. In Arabidopsis thaliana, lateral root founder cells originate from pericycle cells adjacent to xylem poles. In order to study the mechanisms of lateral root development, a population of Arabidopsis GAL4-GFP enhancer trap lines were screened and two lines were isolated with GAL4 expression in root xylem-pole pericycle cells (J0121), i.e. in cells competent to become lateral root founder cells, and in young lateral root primordia (J0192). These two enhancer trap lines are very useful tools with which to study the molecular and cellular bases of lateral root development using targeted gene expression. These lines were used for genetic ablation experiments by targeting the expression of a toxin-encoding gene. Moreover, the molecular bases of the enhancer trap expression pattern were characterized. These results suggest that the lateral-root-specific GAL4 expression pattern in J0192 is due to a strong enhancer in the promoter of the LOB-domain protein gene LBD16.

Keywords: Genetic ablation; lateral root founder cells; lateral root primordia; patterning; pericycle.
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