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JXB Advance Access published online on July 12, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri241
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received March 18, 2005
Accepted June 2, 2005

RESEARCH PAPER

A versatile promoter for the expression of proteins in glandular and non-glandular trichomes from a variety of plants

Gloria Gutiérrez-Alcalá 1, Leticia Calo 1, Florence Gros 2, Jean-Claude Caissard 2, Cecilia Gotor 1, and Luis C. Romero 1*

1 Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, CSIC-Universidad de Sevilla, Avda Américo Vespucio, 49, 41092-Sevilla, Spain
2 Laboratoire BVpam (Biotechnologies Végétales, plantes aromatiques et médicinales), Faculté des Sciences et Techniques, Université Jean Monnet, 23, rue du Docteur Michelon, F-42023 Saint-Etienne Cedex 02, France

* To whom correspondence should be addressed.
Luis C. Romero, E-mail: lromero{at}ibvf.csic.es


  Abstract

A DNA regulatory fragment was isolated from the promoter region of the OASA1 gene, encoding the cytosolic O-acetylserine(thiol)lyase enzyme that is highly expressed in Arabidopsis thaliana trichomes. This DNA fragment has been named an ATP fragment and comprises 1435 bp of the genomic region upstream of the OASA1 gene and 375 bp of the transcriptional initiation start site containing the first intron of the gene. The ATP fragment, fused to the green fluorescent protein (GFP) and {beta}-glucuronidase (GUS) reporter genes, is able to drive high-level gene expression in A. thaliana trichomes. Deletion analysis of the ATP fragment determined that the region from -266 to -66 contains regulatory elements required for trichome expression. In addition, the region from +112 to +375, comprising the first intronic region of the gene, is also essential for trichome gene expression. Expression of the full-length ATP fragment in tobacco and peppermint shows that this fragment is also able to drive expression in glandular trichomes and suggests additional biotechnological applications for this promoter.

Keywords: Arabidopsis; confocal microscopy; peppermint; tobacco.
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