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JXB Advance Access published online on August 16, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri260
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org
Received November 2, 2004
Accepted July 4, 2005

RESEARCH PAPER

Overexpression in transgenic tobacco reveals different roles for the rice homeodomain gene OsBIHD1 in biotic and abiotic stress responses

Hongli Luo 1, Fengming Song 2*, and Zhong Zheng 3

1 Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China; Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China; Present address: Institute of Biotechnology, Hainan University, Haikou, Hainan 570000, PR China
2 Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China; Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China; State Key Laboratory for Rice Biology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China
3 Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China; Department of Plant Protection, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029, PR China

* To whom correspondence should be addressed.
Fengming Song, E-mail: fmsong{at}zju.edu.cn


  Abstract

The rice OsBIHD1 gene encodes a transcriptional factor belonging to the homeodomain class. It had previously been shown to be activated by treatment with benzothiadiazole, a chemical inducer of disease resistance, and in an incompatible interaction between rice and the blast fungus. To allow a better understanding of the function of OsBIHD1 in plant disease resistance response, the OsBIHD1 gene in tobacco was overexpressed by Agrobacterium-mediated leaf disc transformation with a construct containing the OsBIHD1 ORF under control of the 35S promoter. Overexpression of the rice OsBIHD1 gene in some of the transgenic tobacco lines led to some morphological abnormalities in the top buds and roots. The transgenic tobacco plants showed an elevated level of defence-related PR-1 gene expression and enhanced disease resistance against infection by tomato mosaic virus, tobacco mosaic virus, and Phytophthora parasitica var. nicotianae. However, the transgenic tobacco plants overexpressing OsBIHD1 showed enhanced sensitivity to salt and oxidative stress as compared with the wild-type plants. The results suggested that the OsBIHD1 protein may be positively involved in activating expression of the defence-related genes in disease resistance responses, and is also important in rice development and abiotic stress tolerance.

Keywords: Disease resistance response; homeodomain; OsBIHD1; oxidative stress tolerance; salt tolerance; transgenic tobacco plant.
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