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JXB Advance Access published online on October 10, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri271
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Published by Oxford University Press [2005] on behalf of the Society for Experimental Biology.
Received April 30, 2005
Accepted July 26, 2005

RESEARCH PAPER

Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress

Antonio J. Castro 1, Christine Carapito 2, Nathalie Zorn 2, Christian Magné 3, Emmanuelle Leize 2, Alain Van Dorsselaer 2, and Christophe Clément 4*

1 Laboratoire de Stress, Défenses et Reproduction des Plantes, URVVC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, BP 1039 Moulin de la Housse, F-51687 Reims cedex 2, France; Present address: Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín (CSIC), C/ Profesor Albareda 1, E-18008 Granada, Spain
2 Laboratoire de Spectrométrie de Masse Bioorganique, UMR ULP/CNRS 7509, École Européenne de Chimie, Polymères et Matériaux, 25 rue Becquerel, F-67087 Strasbourg cedex 2, France
3 Laboratoire de Stress, Défenses et Reproduction des Plantes, URVVC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, BP 1039 Moulin de la Housse, F-51687 Reims cedex 2, France; Present address: LEBHAM, UPRES EA 3877, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Technopole Brest Iroise, Place Nicolas Copernic, F-29280 Plouzané, France
4 Laboratoire de Stress, Défenses et Reproduction des Plantes, URVVC UPRES EA 2069, Université de Reims Champagne Ardenne, UFR Sciences, BP 1039 Moulin de la Housse, F-51687 Reims cedex 2, France

* To whom correspondence should be addressed.
Christophe Clément, E-mail: christophe.clement{at}univ-reims.fr


  Abstract

Two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine for the first time the effect of a herbicide (flumioxazin) on a crop species (Vitis vinifera L.) at the proteome level. Examination of 2-D maps derived from chemically stressed tissues revealed the presence of 33 spots displaying a differential expression pattern. The presence of stress responsive proteins in the different plant organs analysed suggests that flumioxazin could act systemically. Among the responsive proteins, some photosynthesis-related proteins, including several fragments of the enzyme Rubisco, were identified. This effect suggests that photosynthesis could be impaired by the herbicide. The induction of several enzymatic antioxidant systems was also observed, probably as a result of an oxidative stress. Moreover, the photorespiration pathway was stimulated, as suggested by the induction of some key enzymes involved in this process. Changes in carbon metabolism-associated proteins presumably reflect altered patterns of carbon flux in response to impaired photosynthesis and an increased need for osmotic adjustment in affected tissues. Finally, plant defences were stimulated as revealed by the induction of a set of proteins belonging to the pathogenesis-related 10 class, suggesting that they could play an essential role in cell defence mechanisms against flumioxazin.

Keywords: De novo sequencing; flumioxazin; grapevine; herbicide stress; PRP-10; two-dimensional electrophoresis.
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