Skip Navigation



JXB Advance Access published online on September 19, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri288
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/421/2925    most recent
eri288v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Lanfermeijer, F. C.
Right arrow Articles by Hille, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lanfermeijer, F. C.
Right arrow Articles by Hille, J.
Agricola
Right arrow Articles by Lanfermeijer, F. C.
Right arrow Articles by Hille, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 31, 2005
Accepted August 12, 2005

RESEARCH PAPER

The products of the broken Tm-2 and the durable Tm-22 resistance genes from tomato differ in four amino acids

Frank C. Lanfermeijer 1*, Jan Warmink 1, and Jacques Hille 1

1 Department Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands

* To whom correspondence should be addressed.
Frank C. Lanfermeijer, E-mail: f.c.lanfermeijer{at}rug.nl


  Abstract

To gain an insight into the processes underlying disease resistance and its durability, the durable Tm-22 resistance gene was compared with the broken Tm-2 resistance gene. The Tm-2 gene of tomato could be isolated via PCR with primers based on the Tm-22 sequence. The Tm-2 gene, like the Tm-22 gene, encodes an 861 amino acid polypeptide, which belongs to the coiled coil/nucleotide binding site/leucine-rich repeat class of resistance proteins. The functionality and the nature of the isolated Tm-2 gene were confirmed by introducing the gene under the control of the 35S promoter into tomato mosaic virus-susceptible tobacco. This transgenic tobacco was crossed with transgenic tobacco plants producing the movement protein (MP)-authenticated MP as the Avr protein of the Tm-2 resistance. The Tm-22 and Tm-2 open reading frames only differ in seven nucleotides, which on a protein level results in four amino acid differences, of which two are located in the nucleotide binding site and two are located in the leucine-rich repeat domain. The small difference between the two proteins suggests a highly similar interaction of these proteins with the MP, which has major implications for the concept of durability. Comparison of the two resistance-conferring alleles (Tm-2 and Tm-22) with two susceptible alleles (tm-2 and lptm-2) allowed discussion of the structure-function relationship in the Tm-2 proteins. It is proposed that the Tm-2 proteins display a partitioning of the leucine-rich repeat domain, in which the N-terminal and C-terminal parts function in signal transduction and MP recognition, respectively.

Keywords: Lycopersicon esculentum; Lycopersicon peruvianum; Tm-2; Tm-22; tomato mosaic virus; plant disease resistance gene; durability; structure-function relationships.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.