JXB Advance Access published online on November 7, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri292
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan
* To whom correspondence should be addressed. A comprehensive analysis of the transcript levels of genes which encode starch-synthesis enzymes is fundamental for the assessment of the function of each enzyme and the regulatory mechanism for starch biosynthesis in source and sink organs. Using quantitative real-time RT-PCR, an examination was made of the expression profiles of 27 rice genes encoding six classes of enzymes, i.e. ADPglucose pyrophosphorylase (AGPase), starch synthase, starch branching enzyme, starch debranching enzyme, starch phosphorylase, and disproportionating enzyme in developing seeds and leaves. The modes of gene expression were tissue- and developmental stage-specific. Four patterns of expression in the seed were identified: group 1 genes, which are expressed very early in grain formation and are presumed to be involved in the construction of fundamental cell machineries, de novo synthesis of glucan primers, and initiation of starch granules; group 2 genes, which are highly expressed throughout endosperm development; group 3 genes, which have transcripts that are low at the onset but which rise steeply at the start of starch synthesis in the endosperm and are thought to play essential roles in endosperm starch synthesis; and group 4 genes, which are expressed scantly, mainly at the onset of grain development, and might be involved in synthesis of starch in the pericarp. The methodology also revealed that the defect in the cytosolic AGPase small subunit2b (AGPS2b) transcription from the AGPS2 gene in endosperm sharply enhanced the expressions of endosperm and leaf plastidial AGPS1, the endosperm cytosolic AGPase large subunit2 (AGPL2), and the leaf plastidial AGPL1.
Received April 6, 2005
Accepted August 18, 2005
RESEARCH PAPER
Expression profiling of genes involved in starch synthesis in sink and source organs of rice
2 National Agricultural Research Center, Joetsu, Niigata 943-0193, Japan
3 Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
4 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 4-1-8, Honcho, Kawaguchi, Saitama 332-0012, Japan; Akita Prefectural University, 241-7 Shimoshinjo-Nakano, Akita 010-0195, Japan
Yasunori Nakamura, E-mail: nakayn{at}akita-pu.ac.jp
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H.-L. Tsai, W.-L. Lue, K.-J. Lu, M.-H. Hsieh, S.-M. Wang, and J. Chen Starch Synthesis in Arabidopsis Is Achieved by Spatial Cotranscription of Core Starch Metabolism Genes Plant Physiology, November 1, 2009; 151(3): 1582 - 1595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Georgelis, J. R. Shaw, and L. C. Hannah Phylogenetic Analysis of ADP-Glucose Pyrophosphorylase Subunits Reveals a Role of Subunit Interfaces in the Allosteric Properties of the Enzyme Plant Physiology, September 1, 2009; 151(1): 67 - 77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tang, H. Xie, Y. Wang, B. Lu, and J. Liang The effect of sucrose and abscisic acid interaction on sucrose synthase and its relationship to grain filling of rice (Oryza sativa L.) J. Exp. Bot., July 1, 2009; 60(9): 2641 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Comparot-Moss and K. Denyer The evolution of the starch biosynthetic pathway in cereals and other grasses J. Exp. Bot., July 1, 2009; 60(9): 2481 - 2492. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Sawada, P. B. Francisco Jr, S. Aihara, Y. Utsumi, M. Yoshida, Y. Oyama, M. Tsuzuki, H. Satoh, and Y. Nakamura Chlorella Starch Branching Enzyme II (BEII) Can Complement the Function of BEIIb in Rice Endosperm Plant Cell Physiol., June 1, 2009; 50(6): 1062 - 1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Radchuk, L. Borisjuk, N. Sreenivasulu, K. Merx, H.-P. Mock, H. Rolletschek, U. Wobus, and W. Weschke Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain Plant Physiology, May 1, 2009; 150(1): 190 - 204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujita, Y. Toyosawa, Y. Utsumi, T. Higuchi, I. Hanashiro, A. Ikegami, S. Akuzawa, M. Yoshida, A. Mori, K. Inomata, et al. Characterization of pullulanase (PUL)-deficient mutants of rice (Oryza sativa L.) and the function of PUL on starch biosynthesis in the developing rice endosperm J. Exp. Bot., March 1, 2009; 60(3): 1009 - 1023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Xu, T. Li, Z. Y. Deng, K. Chong, Y. Xue, and T. Wang Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains Plant Physiology, October 1, 2008; 148(2): 908 - 925. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Satoh, K. Shibahara, T. Tokunaga, A. Nishi, M. Tasaki, S.-K. Hwang, T. W. Okita, N. Kaneko, N. Fujita, M. Yoshida, et al. Mutation of the Plastidial {alpha}-Glucan Phosphorylase Gene in Rice Affects the Synthesis and Structure of Starch in the Endosperm PLANT CELL, July 1, 2008; 20(7): 1833 - 1849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cossegal, P. Chambrier, S. Mbelo, S. Balzergue, M.-L. Martin-Magniette, A. Moing, C. Deborde, V. Guyon, P. Perez, and P. Rogowsky Transcriptional and Metabolic Adjustments in ADP-Glucose Pyrophosphorylase-Deficient bt2 Maize Kernels Plant Physiology, April 1, 2008; 146(4): 1553 - 1570. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujita, M. Yoshida, T. Kondo, K. Saito, Y. Utsumi, T. Tokunaga, A. Nishi, H. Satoh, J.-H. Park, J.-L. Jane, et al. Characterization of SSIIIa-Deficient Mutants of Rice: The Function of SSIIIa and Pleiotropic Effects by SSIIIa Deficiency in the Rice Endosperm Plant Physiology, August 1, 2007; 144(4): 2009 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Hamblin, M. G. Salas Fernandez, M. R. Tuinstra, W. L. Rooney, and S. Kresovich Sequence Variation at Candidate Loci in the Starch Metabolism Pathway in Sorghum: Prospects for Linkage Disequilibrium Mapping Crop Sci., July 16, 2007; 47(S2): S-125 - S-134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Georgelis, E. L. Braun, J. R. Shaw, and L. C. Hannah The Two AGPase Subunits Evolve at Different Rates in Angiosperms, yet They Are Equally Sensitive to Activity-Altering Amino Acid Changes When Expressed in Bacteria PLANT CELL, May 1, 2007; 19(5): 1458 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yamakawa, T. Hirose, M. Kuroda, and T. Yamaguchi Comprehensive Expression Profiling of Rice Grain Filling-Related Genes under High Temperature Using DNA Microarray Plant Physiology, May 1, 2007; 144(1): 258 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Rosti, H. Rudi, K. Rudi, H.-G. Opsahl-Sorteberg, B. Fahy, and K. Denyer The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves J. Exp. Bot., November 1, 2006; 57(14): 3619 - 3626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Fujita, M. Yoshida, N. Asakura, T. Ohdan, A. Miyao, H. Hirochika, and Y. Nakamura Function and Characterization of Starch Synthase I Using Mutants in Rice Plant Physiology, March 1, 2006; 140(3): 1070 - 1084. [Abstract] [Full Text] [PDF]
|
|