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JXB Advance Access published online on November 1, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri301
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 3, 2005
Accepted September 1, 2005

RESEARCH PAPER

Increased sensitivity to salt stress in an ascorbate-deficient Arabidopsis mutant

Chenghong Huang 1, Wenliang He 1, Jinkui Guo 2, Xuexiang Chang 1, Peixi Su 1, and Lixin Zhang 3*

1 Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China
2 School of Life Sciences, Lanzhou University, Lanzhou, 730000, China
3 Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou, 730000, China; School of Life Sciences, Lanzhou University, Lanzhou, 730000, China

* To whom correspondence should be addressed.
Lixin Zhang, E-mail: zhanglixin{at}lzu.edu.cn


  Abstract

The Arabidopsis thaliana ascorbate-deficient vtc-1 mutant has only 30% ascorbate contents of the wild type (WT). This ascorbate-deficient mutant was used here to study the physiological roles of ascorbate under salt stress in vivo. Salt stress resulted in a more significant decrease in CO2 assimilatory capacity in the vtc-1 mutant than in the WT. Photosystem II function in the Arabidopsis vtc-1 mutant also showed an increased sensitivity to salt stress. Oxidative stress, indicated by the hydrogen peroxide content, increased more dramatically in the vtc-1 mutant than in the WT under salt stress. To clarify the reason for the increased oxidative stress in the vtc-1 mutant, the contents of small antioxidant compounds and the activities of several antioxidant enzymes in the ascorbate-glutathione cycle were measured. Despite an elevated glutathione pool in the vtc-1 mutant, the ascorbate contents and the reduced form of ascorbate decreased very rapidly under salt stress. These results showed that the activities of MDAR and DHAR were lower in the vtc-1 mutant than in the WT under salt stress. Thus, low intrinsic ascorbate and an impaired ascorbate-glutathione cycle in the vtc-1 mutant under salt stress probably induced a dramatic decrease in the reduced form of ascorbate, which resulted in both enhanced ROS contents and decreased NPQ in the vtc-1 mutant.

Keywords: Arabidopsis thaliana; ascorbate-glutathione cycle; oxidative stress; photosystem II; vtc-1.
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