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JXB Advance Access published online on November 1, 2005
Journal of Experimental Botany, doi:10.1093/jxb/eri317
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 4, 2005
Accepted September 20, 2005

RESEARCH PAPER

OsZIP4, a novel zinc-regulated zinc transporter in rice

Yasuhiro Ishimaru 1, Motofumi Suzuki 1, Takanori Kobayashi 2, Michiko Takahashi 1, Hiromi Nakanishi 3, Satoshi Mori 3, and Naoko K. Nishizawa 2*

1 Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2 Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Corporation, Tokyo, Japan
3 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

* To whom correspondence should be addressed.
Naoko K. Nishizawa, E-mail: annaoko{at}mail.ecc.u-tokyo.ac.jp


  Abstract

Zinc (Zn) is an essential element for the normal growth of plants but information is scarce on the mechanisms whereby Zn is transported in rice (Oryza sativa L.) plants. Four distinct genes, OsZIP4, OsZIP5, OsZIP6, and OsZIP7 that exhibit sequence similarity to the rice ferrous ion transporter, OsIRT1, were isolated. Microarray and northern blot analysis revealed that OsZIP4 was highly expressed under conditions of Zn deficiency in roots and shoots. Real-time-PCR revealed that the OsZIP4 transcripts were more abundant than those of OsZIP1 or OsZIP3 in Zn-deficient roots and shoots. OsZIP4 complemented a Zn-uptake-deficient yeast (Saccharomyces cerevisiae) mutant, {Delta}zrt1,{Delta}zrt2, indicating that OsZIP4 is a functional transporter of Zn. OsZIP4-synthetic green fluorescent protein (sGFP) fusion protein was transiently expressed in onion epidermal cells localized to the plasma membrane. In situ hybridization analysis revealed that OsZIP4 in Zn-deficient rice was expressed in shoots and roots, especially in phloem cells. Furthermore, OsZIP4 transcripts were detected in the meristem of Zn-deficient roots and shoots. These results suggested that OsZIP4 is a Zn transporter that may be responsible for the translocation of Zn within rice plants.

Keywords: IRT1; meristem; phloem; rice; zinc; ZIP4.
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