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JXB Advance Access published online on December 5, 2005
Journal of Experimental Botany, doi:10.1093/jxb/erj024
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received June 24, 2005
Accepted October 21, 2005

RESEARCH PAPER

A peroxidase gene expressed during early developmental stages of the parasitic plant Orobanche ramosa

Clara Isabel González-Verdejo 1 *, Xabier Barandiaran 2, Maria Teresa Moreno 3, José Ignacio Cubero 1, and Antonio Di Pietro 1

1 Departamento de Genética, Universidad de Córdoba, Campus de Rabanales C5, E-14071 Córdoba, Spain
2 Dominion Biotecnologia, C/ Josefa Valcárcel 3-5, E-28027 Madrid, Spain
3 CIFA, Alameda del Obispo, IFAPA, Area de Mejora y Biotecnología, Apdo. 3092, E-14080 Córdoba, Spain

* To whom correspondence should be addressed.
Clara Isabel González-Verdejo, E-mail: clara2gonzalez{at}yahoo.com


  Abstract

Broomrapes (Orobanche spp.) are holoparasitic weeds that cause devastating losses in many economically important crops. The molecular mechanisms that control the early stages of host infection in Orobanche are poorly understood. In the present study, the role of peroxidase has been examined during pre-infection growth and development of O. ramosa, using an in vitro model system. Peroxidase activity was histochemically localized at the tips of actively growing radicles and nascent attachment organs. Addition of exogenous catalase resulted in a significant reduction in the apical growth rate of the radicle. The prx1 gene encoding a putative class III peroxidase was cloned from a cDNA library of O. ramosa and was found to be expressed specifically during the early stages of the parasitic life cycle. The exogenous addition of sucrose resulted in significantly reduced prx1 transcript levels and in a dramatic change in radicle development from polarized apical growth to isotropic growth and the formation of tubercle-like structures. The results indicate an important role of peroxidases during the early parasitic stages of Orobanche.

Keywords: Attachment organ; catalase; cDNA library; germination; in vitro culture; polarized growth; sucrose.
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