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JXB Advance Access published online on November 29, 2005
Journal of Experimental Botany, doi:10.1093/jxb/erj025
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© The Author [2005]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 14, 2005
Accepted October 21, 2005

RESEARCH PAPER

Identification of a mitochondrial ATP synthase small subunit gene (RMtATP6) expressed in response to salts and osmotic stresses in rice (Oryza sativa L.)

Xinxin Zhang 1, Tetsuo Takano 2, and Shenkui Liu 3 *

1 Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin 150040, PR China; Asian Natural Environment Science Center (ANESC), The University of Tokyo, 1-1-1 Midori-cho, Nishi-tokyo, Tokyo 188-0002, Japan
2 Asian Natural Environment Science Center (ANESC), The University of Tokyo, 1-1-1 Midori-cho, Nishi-tokyo, Tokyo 188-0002, Japan
3 Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin 150040, PR China

* To whom correspondence should be addressed.
Shenkui Liu, E-mail: shenkuiliu{at}nefu.edu.cn


  Abstract

Large areas of northern China have alkaline soil due to the accumulation of sodium carbonates (NaHCO3, Na2CO3). To understand better how plants can tolerate alkaline soil, a cDNA library was prepared from rice (Oryza sativa L.) roots grown in the presence of NaHCO3 stress. A cDNA clone isolated from this library was identified by a homology search as a mitochondrial ATP synthase 6 kDa subunit gene (RMtATP6; GenBank accession nos AB055076, BAB21526). In transformed yeast and tobacco protoplasts, the RMtATP6 protein was localized in mitochondria using the green fluorescent protein (GFP) marker. Analysis of RMtATP6 mRNA levels suggested that the expression of this gene was induced by stress from sodium carbonates and other sodium salts. Transgenic tobacco overexpressing the RMtATP6 gene had greater tolerance to salt stress at the seedling stage than untransformed tobacco. Among the other genes for F1F0-ATPase of rice, some were found to be up-regulated by some environmental stresses and some were not. These data suggest that the RMtATP6 protein acts as a subunit of ATP synthase, and is expressed in response to stress from several salts, with the other genes coding for the subunits of the same ATP-synthase.

Keywords: Carbonate stress; cloning; GFP; mitochondria; rice mitochondrial ATP synthase 6 kDa subunit; transgenic; yeast.
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