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JXB Advance Access published online on February 10, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj071
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and the Society for Experimental Biology are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org
Received May 26, 2005
Accepted November 23, 2005

RESEARCH PAPER

SECRET AGENT and SPINDLY have overlapping roles in the development of Arabidopsis thaliana L. Heyn

Lynn M. Hartweck 1, Ruth K. Genger 2, William M. Grey 1, and Neil E. Olszewski 1 *

1 Department of Plant Biology, University of Minnesota, 250 Biological Sciences Center, 1445 Gortner Ave., St Paul, MN 55108, USA
2 Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison, WI 53706, USA

* To whom correspondence should be addressed.
Neil E. Olszewski, E-mail: neil{at}cbs.umn.edu


  Abstract

O-GlcNAc transferase (OGT) catalyses transfer of GlcNAc (N-acetylglucosamine) to serine or threonine of proteins. The Arabidopsis OGTs, SECRET AGENT (SEC) and SPINDLY (SPY) have overlapping functions during gametogenesis and embryogenesis. SPY functions in a number of processes including circadian, light, and gibberellin (GA) responses. The role of SEC in plant development and GA signalling was investigated by determining the phenotypes of sec-1 and sec-2 plants and the expression pattern of SEC. Similar to SPY, SEC transcripts were ubiquitous. Although there is no evidence of transcript-level regulation by other factors, SEC mRNA levels are elevated in spy plants and SPY mRNA levels are elevated in sec plants. sec-1 and sec-2 plants exhibited few of the defects observed in spy plants and had wild-type GA responses. Compared with wild type, sec plants produced leaves at a reduced rate. Haplo-insufficiency at SEC in a spy ga1 double mutant background suppressed spy during germination and enhanced the production of ovaries with four carpels by spy. By contrast, SPY haplo-insufficiency in a sec ga1 double mutant background caused a novel phenotype, production of a proliferation of pin-like structures instead of a floral shoot. These results are consistent with SEC function overlapping with SPY for leaf production and reproductive development.

Keywords: Flowering time; O-GlcNAc; O-glycosylation; shoot apical meristem.
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