JXB Advance Access published online on March 17, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj111
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1 Laboratory of Plant Nutritional Genetics and Root Biology Center, College of Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China
* To whom correspondence should be addressed. The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 µM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H+-ATPase in soybean roots.
Received June 25, 2005
Accepted December 16, 2005
RESEARCH PAPER
Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation
Hong Shen 1,
Jianhong Chen 1,
Zhanyi Wang 1,
Cunyi Yang 1,
Takara Sasaki 2,
Yoko Yamamoto 2,
Hideaki Matsumoto 2,
and
Xiaolong Yan 1 *
2 Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710-0046, Japan
Xiaolong Yan, E-mail: xlyan{at}scau.edu.cn
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