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JXB Advance Access published online on May 23, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj153
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received August 17, 2005
Accepted January 31, 2006

RESEARCH PAPER

The contribution of extensin network formation to rapid, hydrogen peroxide-mediated increases in grapevine callus wall resistance to fungal lytic enzymes

J. M. Ribeiro 1, C. Silva Pereira 1, N. C. Soares 1, A. M. Vieira 2, J. A. Feijó 2, and P. A. Jackson 1 *

1 Instituto de Tecnologia Química e Biológica, Apartado 127, 2781-901 Oeiras, Portugal
2 Instituto Gulbenkian de Ciências, Apartado 14 2781-901 Oeiras, Portugal

* To whom correspondence should be addressed.
P. A. Jackson, E-mail: Phil{at}itqb.unl.pt


  Abstract

Grapevine (Vitis vinifera cv. Touriga) callus cell walls contain a high level of the monomeric extensin, GvP1. Hydrogen peroxide stimulus of these cultures causes the rapid loss of monomeric GvP1, concomitant with marked increases in insoluble GvP1 amino acids and wall resistance to digestion by fungal lytic enzymes. JIM11 immunolocalization studies indicated that monomeric and network GvP1 were evenly distributed in the callus cell wall. These primary cell walls were used to investigate the specific contribution of extensin and other ionically bound cell-wall proteins to hydrogen peroxide-mediated increases in resistance to fungal lytic enzymes. This was performed by removing ionically-bound proteins and assaying for hydrogen peroxide-enhanced resistance after the addition of selected protein fractions. The results indicate that hydrogen peroxide-induced increases in resistance to digestion by fungal lytic enzymes require a co-operative action between network extensin formation and the electrostatic interaction of additional wall proteins with the extracellular matrix.

Keywords: Disease resistance; extensin network; fungal lytic enzymes; primary cell wall; protoplasts.
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