JXB Advance Access published online on May 10, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj154
1 Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n, 46022-Valencia, Spain
* To whom correspondence should be addressed. Based on its compact habit, Micro-Tom, a dwarf cultivar of tomato (Solanum lycopersicum L.), has been proposed as a preferred variety to carry out molecular research in tomato. This cultivar, however, is poorly characterized. It is shown here that Micro-Tom has mutations in the SELF-PRUNING (SP) and DWARF (D) genes. In addition to this, it is also shown that Micro-Tom harbours at least two independently segregating resistance loci to the plant pathogen Cladosporium fulvum. The presence of the self-pruning mutation in Micro-Tom, that generates a determinate phenotype, was confirmed by crossing and sequence analysis. It was also found that Micro-Tom has a mutation in the DWARF gene (d) that leads to mis-splicing and production of at least two shorter mRNAs. The d mutation is predicted to generate truncated DWARF protein. The d sequence defect co-segregates with dark-green and rugose leaves, characteristics of brassinosteroid biosynthesis mutants. Micro-Tom also carries at least another mutation producing internode length reduction that affects plant height but not active gibberellin (GA) levels, which were similar in dwarf and tall Micro-TomxSeverianin segregants. GAs and brassinosteroids act synergistically in Micro-Tom, and the response to GA depends on brassinosteroids because the elongation of internodes was at least six times higher when GA3 was applied simultaneously with brassinolide. A novel variety, Micro-0 that is fully susceptible to C. fulvum and almost as dwarf as Micro-Tom, has been generated from the cross of Cf0xMicro-Tom. This line represents a valuable resource for future analysis of Cf resistance genes through breeding or transformation.
Received September 13, 2005
Accepted February 6, 2006
RESEARCH PAPER
Genetic and physiological characterization of tomato cv. Micro-Tom
Esmeralda Martí 1,
Carmina Gisbert 2,
Gerard J. Bishop 3,
Mark S. Dixon 4,
and
José L. García-Martínez 1 *
2 Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Avda de los Naranjos s/n, 46022-Valencia, Spain; Present address: Centro de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Camino de Vera s/n, 460022 Valencia, Spain
3 Division of Biology, Imperial College London, Wye Campus, Ashford, Kent TN25 5AH, UK
4 School of Biological Sciences, University of Southampton, Southampton SO16 7PX, UK
José L. García-Martínez, E-mail: jlgarcim{at}ibmcp.upv.es
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