JXB Advance Access published online on May 23, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj158
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1 Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University, N21W10, Kita-ku, Sapporo, 001-0021 Japan
* To whom correspondence should be addressed. Plants have developed several strategies for coping with phosphorus (P) deficiency. However, the details of the regulation of gene expression of adaptations to low P are still unclear. Using a cDNA microarray, transcriptomic analyses were carried out of the rice genes regulated by P deficiency and P re-supply to P-deficient plants. The OsPI1 gene, which was isolated as the most significant up-regulated gene under -P conditions, was also the most significant down-regulated gene following P re-supply. Many starch metabolism-related genes, as well as several genes for Pi-liberating enzymes, were up-regulated by -P treatment, suggesting a homeostatic contribution to the Pi concentration in leaf tissues. mRNAs for glucanases were also induced by P re-supply: these are suspected to play a role in loosening the cell wall compounds. Most of the genes up-regulated by -P treatment were down-regulated by P re-supply, suggesting that their responses were specific to -P conditions. Conversely, the number of genes up-regulated by P re-supply was also larger following P re-supply than in the -P condition. It is proposed that the genes up-regulated by P re-supply play an important role in P acquisition by P-deficient plants.
Received August 24, 2005
Accepted February 3, 2006
RESEARCH PAPER
Transcriptomic analysis indicates putative metabolic changes caused by manipulation of phosphorus availability in rice leaves
Jun Wasaki 1 *,
Takuro Shinano 1,
Kazuki Onishi 2,
Ryoma Yonetani 2,
Junshi Yazaki 3,
Fumiko Fujii 3,
Kanako Shimbo 4,
Masahiro Ishikawa 3,
Zempei Shimatani 4,
Yuko Nagata 3,
Akiko Hashimoto 4,
Tomoya Ohta 3,
Yuki Sato 3,
Chikako Miyamoto 3,
Sachiko Honda 3,
Keiichi Kojima 5,
Takuji Sasaki 3,
Naoki Kishimoto 3,
Shoshi Kikuchi 3,
and
Mitsuru Osaki 2
2 Graduate School of Agriculture, Hokkaido University, N9W9, Kita-ku, Sapporo, 060-8589 Japan
3 National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, Ibaraki, 305-8602 Japan
4 Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854 Japan
5 Hitachi Software Engineering, 4-12-7, Higashi-Shinagawa, Shinagawa, Tokyo, 140-0002 Japan
Jun Wasaki, E-mail: junw{at}chem.agr.hokudai.ac.jp
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