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JXB Advance Access published online on April 4, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj175
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received December 13, 2005
Accepted March 1, 2006

Oxygen Metabolism Special Issue Article

Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress*

Juan B. Barroso 1, Francisco J. Corpas 2 *, Alfonso Carreras 1, María Rodríguez-Serrano 2, Francisco J. Esteban 1, Ana Fernández-Ocaña 1, Mounira Chaki 1, María C. Romero-Puertas 2, Raquel Valderrama 1, Luisa M. Sandalio 2, and Luis A. del Río 2

1 Grupo de Señalización Molecular y Sistemas Antioxidantes en Plantas, Unidad Asociada al CSIC (Estación Experimental del Zaidín), Área de Bioquímica y Biología Molecular, Universidad de Jaén, E- 23071 Jaén, Spain
2 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, E-18080 Granada, Spain

* To whom correspondence should be addressed.
Francisco J. Corpas, E-mail: avier.corpas{at}eez.csic.es


  Abstract

S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 µM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress.

Keywords: Abiotic stress; collenchyma; formaldehyde dehydrogenase; nitric oxide; reactive nitrogen species; RNS; S-nitrosoglutathione; S-nitrosoglutathione reductase; signalling.

*Sequence data from this article have been deposited with the EMBL/GenBank data libraries under accession number DQ084382.


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