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JXB Advance Access published online on May 23, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj176
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received October 11, 2005
Accepted February 27, 2006

RESEARCH PAPER

Effects of simultaneous expression of heterologous genes involved in phytochelatin biosynthesis on thiol content and cadmium accumulation in tobacco plants

Adam Wawrzynski 1, Edyta Kopera 1, Anna Wawrzynska 1, Jolanta Kaminska 1, Wojciech Bal 1, and Agnieszka Sirko 1 *

1 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5A, 02-106 Warsaw, Poland

* To whom correspondence should be addressed.
Agnieszka Sirko, E-mail: asirko{at}ibb.waw.pl


  Abstract

Transgenic tobacco (Nicotiana tabacum cv. LA Burley 21) lines expressing three genes encoding enzymes thought to be critical for the efficient production of phytochelatins, (i) serine acetyltransferase (EC 2.3.1.30) involved in the production of O-acetylserine, the cysteine precursor, (ii) {gamma}-glutamylcysteine synthetase (EC 6.3.2.2) involved in the production of {gamma}-glutamylcysteine, the precursor of glutathione, and (iii) phytochelatin synthase (EC 2.3.2.15), were obtained and analysed for non-protein thiol content and cadmium accumulation. After a 3 week exposure to 15 µM CdCl2, plants expressing transgenes (either separately or in combination) had increased cadmium concentration in roots but not in shoots compared with the wild type. Nearly all transgenic lines analysed had more non-protein thiols than the wild type. The greatest effects (about 8-fold elevation of thiols) were found in one of the lines simultaneously expressing the three transgenes. Despite the fact that a multi-transgene strategy described in this work resulted in a strong increase in the levels of several classes of non-protein thiols in transgenic plants, other factors appeared to restrict cadmium accumulation in shoots.

Keywords: Cadmium; glutathione; phytochelatin; thiols; tobacco; transgenic plants.
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S. Wojas, S. Clemens, J. Hennig, A. Sklodowska, E. Kopera, H. Schat, W. Bal, and D. M. Antosiewicz
Overexpression of phytochelatin synthase in tobacco: distinctive effects of AtPCS1 and CePCS genes on plant response to cadmium
J. Exp. Bot., May 7, 2008; (2008) ern092v1.
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