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JXB Advance Access published online on May 12, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj191
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received January 24, 2006
Accepted March 13, 2006

Oxygen Metabolism ROS and Redox Signalling in Plants Special Issue

Antioxidative enzymes from chloroplasts, mitochondria, and peroxisomes during leaf senescence of nodulated pea plants

José M. Palma 1 *, Ana Jiménez 2, Luisa M. Sandalio 1, Francisco J. Corpas 1, Marianne Lundqvist 2, Manuel Gómez 3, Francisca Sevilla 2, and Luis A. del Río 1

1 Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, Spain
2 Departamento de Biología del Estrés y Patología Vegetal, Centro de Edafología y Biología Aplicada del Segura, CSIC, Apartado 195, 30080 Murcia, Spain
3 Departamento de Agroecología y Protección Vegetal, Estación Experimental del Zaidín, CSIC, Apartado 419, 18080 Granada, Spain

* To whom correspondence should be addressed.
José M. Palma, E-mail: jmpalma{at}eez.csic.es


  Abstract

In this work the influence of the nodulation of pea (Pisum sativum L.) plants on the oxidative metabolism of different leaf organelles from young and senescent plants was studied. Chloroplasts, mitochondria, and peroxisomes were purified from leaves of nitrate-fed and Rhizobium leguminosarum-nodulated pea plants at two developmental stages (young and senescent plants). In these cell organelles, the activity of the ascorbate-glutathione cycle enzymes ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR), and the ascorbate and glutathione contents were determined. In addition, the total superoxide dismutase (SOD) activity, the pattern of mitochondrial and peroxisomal NADPH-generating dehydrogenases, some of the peroxisomal photorespiratory enzymes, the glyoxylate cycle and oxidative metabolism enzymes were also analysed in these organelles. Results obtained on the metabolism of cell organelles indicate that nodulation with Rhizobium accelerates senescence in pea leaves. A considerable decrease of the ascorbate content of chloroplasts, mitochondria, and peroxisomes was found, and in these conditions a metabolic conversion of leaf peroxisomes into glyoxysomes, characteristic of leaf senescence, took place.

Keywords: Ascorbate-glutathione cycle; chloroplasts; mitochondria; peroxisomes; root nodules; senescence.
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