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JXB Advance Access published online on June 23, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erj207
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© 2006 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received January 9, 2006
Accepted March 16, 2006

RESEARCH PAPER

The influence of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) on potato tuber metabolism

Mohammad-Reza Hajirezaei 1 *, Sophia Biemelt 2, Martin Peisker 1, Anna Lytovchenko 3, Alisdair R. Fernie 3, and Uwe Sonnewald 2

1 Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, D-06466 Gatersleben, Germany
2 Department of Biochemistry, Straudtstrasse 5, Friedrich-Alexander Universität Erlangen-Nuernberg, D-91058 Erlangen, Germany
3 Max-Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, D-14476 Potsdam-Golm, Germany

* To whom correspondence should be addressed.
Mohammad-Reza Hajirezaei, E-mail: mohammad{at}ipk-gatersleben.de


  Abstract

The aim of this work was to investigate the importance of cytosolic phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPC) in potato carbohydrate metabolism. For this purpose, the cytosolic isoform of phosphorylating GAPC was cloned and used for an antisense approach to generate transgenic potato plants that exhibited constitutively decreased GAPDH activity. Potato lines with decreased activities of phosphorylating GAPC exhibited no major changes in either whole-plant or tuber morphology. However, the levels of 3-phosphoglycerate were decreased in leaves of the transformants. A broad metabolic phenotyping of tubers from the transformants revealed an increase in sucrose and UDPglucose content, a decrease in the glycolytic intermediates 3-phosphoglycerate and phosphoenolpyruvate but little change in the levels of other metabolites. Moreover, the transformants displayed no differences in cold sweetening with respect to the wild type. Taken together these data suggest that phosphorylating GAPC plays only a minor role in the regulation of potato metabolism. The results presented here are discussed in relation to current models regarding primary metabolism in the potato tuber parenchyma.

Keywords: Antisense; carbohydrate metabolism; GAPDH; potato tuber.
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