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JXB Advance Access published online on July 4, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl015
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received November 18, 2005
Accepted April 11, 2006

FOCUS PAPER

Quantification of cuticular permeability in genetically modified plants

Gerhard Kerstiens 1 *, Lukas Schreiber 2, and Klaus J. Lendzian 3

1 Lancaster Environment Centre/Biological Sciences, Lancaster University, Lancaster LA1 4YQ, UK
2 Ecophysiology of Plants, IZMB - Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany
3 Lehrstuhl für Botanik, Technische Universität München, Wissenschaftszentrum Weihenstephan, Department Biowissenschaftliche Grundlagen, Am Hochanger 4, D-85350 Freising, Germany

* To whom correspondence should be addressed.
Gerhard Kerstiens, E-mail: g.kerstiens{at}lancaster.ac.uk


  Abstract

More and more studies on genetically modified plants are identifying parts of the genetic code with putative involvement in creating the cuticular barrier. Unfortunately, many of these studies suffer from the inadequacy of the chosen methods to quantify, in a reasonably unambiguous way, if and how the efficacy of the cuticular barrier is affected by the genetic change. A short overview of relevant findings is given and a more stringent experimental approach to quantifying effects on cuticular permeability in genetically modified plants proposed.

Keywords: Arabidopsis thaliana; biomembranes; cuticle; cuticular waxes; functional genetics; Hedera helix; transpiration; water permeability.
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