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JXB Advance Access published online on July 14, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl045
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© 2006 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received February 6, 2006
Accepted April 29, 2006

RESEARCH PAPER

Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis

Steven Footitt 1, Julietta Marquez 2, Heike Schmuths 2, Alison Baker 3, Frederica L. Theodoulou 1, and Michael Holdsworth 2 *

1 Crop Performance and Improvement Division, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK
2 Division of Agricultural and Environmental Sciences, School of Biosciences, University of Nottingham, Nottingham LE12 5RD, UK
3 Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, UK

* To whom correspondence should be addressed.
Michael Holdsworth, E-mail: michael.holdsworth{at}nottingham.ac.uk


  Abstract

Comparative physiological analysis of mutant Arabidopsis seeds under defined environmental conditions was used to analyse the relative contributions of components of peroxisomal beta-oxidation in the control of seed germination potential. The COMATOSE (CTS) and KAT2 loci were shown to play essential roles in regulating germination and establishment potentials, whereas LACS6 and LACS7 loci only influenced establishment following germination. The viability and desiccation tolerance of three different mutant alleles of CTS were shown to be intermediate between that of dormant and non-dormant wild-type seeds. Analysis of ttg-1 cts-1 double mutant seeds demonstrated that the cts lesion did not influence after-ripening capacity. These data demonstrate the importance of peroxisomal beta-oxidation in the control of germination potential, but suggest that breakdown of stored lipid is not an important prerequisite for germination. A function is suggested for CTS following after-ripening within pathways related to the progression of germination prior to radicle emergence.

Keywords: Arabidopsis; beta-oxidation; COMATOSE; gene expression; germination; peroxisome; physiological genetics.
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