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JXB Advance Access published online on August 17, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl062
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 20, 2006
Accepted May 23, 2006

RESEARCH PAPER

Comparative cDNA-AFLP analysis of Cd-tolerant and -sensitive genotypes derived from crosses between the Cd hyperaccumulator Arabidopsis halleri and Arabidopsis lyrata ssp. petraea

Adrian Radu Craciun 1, Mikael Courbot 2, Fabienne Bourgis 3, Pietrino Salis 1, Pierre Saumitou-Laprade 4, and Nathalie Verbruggen 1 *

1 Laboratoire de Physiologie et Génétique Moléculaire des Plantes, Université Libre de Bruxelles, Campus Plaine CP242, B-1050, Bruxelles, Belgium
2 Laboratoire de Physiologie et Génétique Moléculaire des Plantes, Université Libre de Bruxelles, Campus Plaine CP242, B-1050, Bruxelles, Belgium; Present address: Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, UK
3 Laboratoire de Physiologie et Génétique Moléculaire des Plantes, Université Libre de Bruxelles, Campus Plaine CP242, B-1050, Bruxelles, Belgium; Present address: Institut de Recherche pour le Développement, 911 Avenue Agropolis, BP 64501; F-34394 Montpellier cedex 5, France
4 Laboratoire de Génétique et Evolution des Populations Végétales, UMR CNRS 8016, FR CNRS 1818, Université de Lille 1, F-59655 Villeneuve d'Ascq Cedex, France

* To whom correspondence should be addressed.
Nathalie Verbruggen, E-mail: nverbru{at}ulb.ac.be


  Abstract

Cadmium (Cd) tolerance seems to be a constitutive species-level trait in Arabidopsis halleri. In order to identify genes potentially implicated in Cd tolerance, a backcross (BC1) segregating population was produced from crosses between A. halleri ssp. halleri and its closest non-tolerant relative A. lyrata ssp. petraea. The most sensitive and tolerant genotypes of the BC1 were analysed on a transcriptome-wide scale by cDNA-amplified fragment length polymorphism (AFLP). A hundred and thirty-four genes expressed more in the root of tolerant genotypes than in sensitive genotypes were identified. Most of the identified genes showed no regulation in their expression when exposed to Cd in a hydroponic culture medium and belonged to diverse functional classes, including reactive oxygen species (ROS) detoxification, cellular repair, metal sequestration, water transport, signal transduction, transcription regulation, and protein degradation, which are discussed.

Keywords: Arabidopsis halleri; cadmium; cDNA-AFLP; heavy metal tolerance; hyperaccumulator.
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