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JXB Advance Access published online on September 6, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl089
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 31, 2006
Accepted June 21, 2006

RESEARCH PAPER

ENOD40 affects elongation growth in tobacco Bright Yellow-2 cells by alteration of ethylene biosynthesis kinetics

Tom Ruttink 1, Kees Boot 2, Jan Kijne 2, Ton Bisseling 3, and Henk Franssen 3 *

1 Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands; Present address: Department of Plant Systems Biology, VIB/Gent University, Technologiepark 927, 9052, Gent, Belgium
2 Institute of Biology, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, The Netherlands
3 Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands

* To whom correspondence should be addressed.
Henk Franssen, E-mail: Henk.Franssen{at}wur.nl


  Abstract

Plant developmental processes are controlled by co-ordinated action of phytohormones and plant genes encoding components of developmental response pathways. ENOD40 was identified as a candidate for such a plant factor with a regulatory role during nodulation. Although its mode of action is poorly understood, several lines of evidence suggest interaction with phytohormone response pathways. This hypothesis was investigated by analysing cytokinin-, auxin-, and ethylene-induced responses on cell growth and cell division in transgenic 35S:NtENOD40 Bright Yellow-2 (BY-2) tobacco cell suspensions. It was found that cell division frequency is controlled by the balance between cytokinin and auxin in wild-type cells and that this regulation is not affected in 35S:NtENOD40 lines. Elongation growth, on the other hand, is reduced upon overexpression of NtENOD40. Analysis of ethylene homeostasis shows that ethylene accumulation is accelerated in 35S:NtENOD40 lines. ENOD40 action can be counteracted by an ethylene perception blocker, indicating that ethylene is a negative regulator of elongation growth in 35S:NtENOD40 cells, and that the NtENOD40-induced response is mediated by alteration of ethylene biosynthesis kinetics.

Keywords: BY-2 cells; elongation growth; ENOD40; ethylene.
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