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JXB Advance Access published online on September 12, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl108
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 4, 2006
Accepted July 3, 2006

RESEARCH PAPER

Sethoxydim affects lipid synthesis and acetyl-CoA carboxylase activity in soybean

Aicha Belkebir 1, Rosine De Paepe 2 *, Antoine Trémolierés 2, Fatiha Aïd 1, and Ghouziel Benhassaine-Kesri 3

1 Physiologie Végétale/LBPO, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne, BP 39, El Alia, Bab Ezzouar, Alger, Algérie
2 Institut de Biotechnologie des Plantes, UMR CNRS 8618, Université Paris-Sud, F-91405 Orsay, France
3 Université Pierre et Marie Curie-Paris 6, FRE 2846, PCMP, Ivry-sur-Seine, F-94200 France

* To whom correspondence should be addressed.
Rosine De Paepe, E-mail: depaepe{at}ibp.u-psud.fr


  Abstract

With rare exceptions, dicot plastids have been reported to contain only a multisubunit (prokaryotic) form of acetyl-coA carboxylase (ACCase), the first committed step of lipid biosynthesis. The sensitivity of most monocots to cyclohexanediones (CHDs) such as sethoxydim, has been shown to be associated with the presence in their plastids of a multifunctional (eukaryotic) form of ACCase. Little is known about the effects of sethoxydim on lipid metabolism and ACCase activity in dicots. Here it is shown that foliar lipid biosynthesis is differentially affected by the herbicide treatment in two dicot species, Nicotiana sylvestris (wild tobacco) and Glycine max (soybean). In N. sylvestris, the total lipid content of neoformed leaves harvested 2 weeks after the sethoxydim treatment was unaffected by doses of up to 10-3 M sethoxydim. In soybean, lipid content decreased by 45% when 10-5 M sethoxydim was used, and this was associated with a 30% reduction in fatty acid synthesis activity. ACCase activity of soybean plastidial preparations was 60% reduced in the presence of sethoxydim, whereas that of N. sylvestris was unaffected. Finally, the presence of a biotinylated 220 kDa polypeptide, corresponding in size to multifunctional ACCase, was observed in soybean plastids. Possible relationships between sensitivity of plastidial soybean ACCase towards sethoxydim, plastidial protein content, and altered de novo lipid biosynthesis in herbicide-treated plants are discussed.

Keywords: Acetyl-CoA carboxylase (ACCase); fatty acid synthesis; plant lipid metabolism; sethoxydim.
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