Skip Navigation



JXB Advance Access published online on September 12, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl111
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Material
Right arrow Supplementary Data
Right arrow All Versions of this Article:
57/14/3601    most recent
erl111v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Herman, E. M.
Right arrow Articles by Livingston, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herman, E. M.
Right arrow Articles by Livingston, D. P., III
Agricola
Right arrow Articles by Herman, E. M.
Right arrow Articles by Livingston, D. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press [2006] on behalf of the Society for Experimental Biology
Received June 12, 2006
Accepted July 5, 2006

RESEARCH PAPER

Additional freeze hardiness in wheat acquired by exposure to -3 °C is associated with extensive physiological, morphological, and molecular changes

Eliot M. Herman 1 *, Kelsi Rotter 2, Ramaswamy Premakumar 3, G. Elwinger 3, Rino Bae 4, Linda Ehler-King 4, Sixue Chen 5, and David P. Livingston III 3

1 Plant Genetics Research Unit, USDA/ARS, Donald Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, USA; Climate Stress Laboratory, USDA/ARS, Beltsville, MD 20705, USA
2 Plant Genetics Research Unit, USDA/ARS, Donald Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, USA
3 USDA/ARS, and North Carolina State University, Raleigh, NC 27695, USA
4 Climate Stress Laboratory, USDA/ARS, Beltsville, MD 20705, USA
5 Proteomics Facility, Donald Danforth Plant Science Center, 975 N. Warson Rd, St Louis, MO 63132, USA

* To whom correspondence should be addressed.
Eliot M. Herman, E-mail: eherman{at}danforthcenter.org


  Abstract

Cold-acclimated plants acquire an additional 3-5 °C increase in freezing tolerance when exposed to -3 °C for 12-18 h before a freezing test (LT50) is applied. The -3 °C treatment replicates soil freezing that can occur in the days or weeks leading to overwintering by freezing-tolerant plants. This additional freezing tolerance is called subzero acclimation (SZA) to differentiate it from cold acclimation (CA) that is acquired at above-freezing temperatures. Using wheat as a model, results have been obtained indicating that SZA is accompanied by changes in physiology, cellular structure, the transcriptome, and the proteome. Using a variety of assays, including DNA arrays, reverse transcription-polymerase chain reaction (RT-PCR), 2D gels with mass spectroscopic identification of proteins, and electron microscopy, changes were observed to occur as a consequence of SZA and the acquisition of added freezing tolerance. In contrast to CA, SZA induced the movement of intracellular water to the extracellular space. Many unknown and stress-related genes were upregulated by SZA including some with obvious roles in SZA. Many genes related to photosynthesis and plastids were downregulated. Changes resulting from SZA often appeared to be a loss of rather than an appearance of new proteins. From a cytological perspective, SZA resulted in alterations of organelle structure including the Golgi. The results indicate that the enhanced freezing tolerance of SZA is correlated with a wide diversity of changes, indicating that the additional freezing tolerance is the result of complex biological processes.

Keywords: Aquaporin; cold acclimation; DNA array; electron microscopy; freeze hardiness; proteome; proteomics; transcriptome; wheat.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 The Plant GenomeHome page
B.C. Koo, B.S. Bushman, and I.W. Mott
Transcripts Associated with Non-Acclimated Freezing Response in Two Barley Cultivars
The Plant Genome, July 1, 2008; 1(1): 21 - 32.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.