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JXB Advance Access published online on September 21, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl119
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received March 12, 2006
Accepted July 14, 2006

Integrated Approaches to Sustain and Improve Plant Production under Drought Stress Special Issue

Comparing regional transcript profiles from maize primary roots under well-watered and low water potential conditions

V. Poroyko 1, W. G. Spollen 2, L. G. Hejlek 3, A. G. Hernandez 4, M. E. LeNoble 3, G. Davis 3, H. T. Nguyen 3, G. K. Springer 2, R. E. Sharp 3, and H. J. Bohnert 5 *

1 Department of Plant Biology and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
2 Department of Computer Science, University of Missouri, Columbia, MO 65211, USA
3 Division of Plant Sciences, University of Missouri, Columbia, MO 65211-0001, USA
4 W. M. Keck Center for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
5 Department of Plant Biology and Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; W. M. Keck Center for Comparative and Functional Genomics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

* To whom correspondence should be addressed.
H. J. Bohnert, E-mail: bohnerth{at}life.uiuc.edu


  Abstract

Regionally distinct elongation responses to water stress in the maize primary root tip have been observed in the past. A genetic basis for such differential responses has been demonstrated. Normalized bar-coded cDNA libraries were generated for four regions of the root tip, 0-3 mm (R1), 3-7 mm (R2), 7-12 mm (R3), and 12-20 mm (R4) from the root apex, and transcript profiles for these regions were sampled. This permitted a correlation between transcript nature and regional location for 15 726 expressed sequence tags (ESTs) that, in approximately equal numbers, derived from three conditions of the root: water stress (water potential: -1.6 MPa) for 5 h and for 48 h, respectively, and well watered (5 h and 48 h combined). These normalized cDNA libraries provided 6553 unigenes. An analysis of the regional representation of transcripts showed that populations were largely unaffected by water stress in R1, correlating with the maintenance of elongation rates under water stress known for R1. In contrast, transcript profiles in regions 2 and 3 diverged in well-watered and water-stressed roots. In R1, transcripts for translation and cell cycle control were prevalent. R2 was characterized by transcripts for cell wall biogenesis and cytoskeleton formation. R3 and R4 shared prevalent groups of transcripts responsible for defence mechanisms, ion transport, and biogenesis of secondary metabolites. Transcripts which were followed for 1, 6, and 48 h of water stress showed distinct region-specific changes in absolute expression and changes in regulated functions.

Keywords: Maize root tip; normalized cDNA libraries; regional transcript profiles; unigene set; water deficit.
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