Skip Navigation



JXB Advance Access published online on October 9, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl136
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
57/14/3747    most recent
erl136v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Borén, M.
Right arrow Articles by Jansson, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Borén, M.
Right arrow Articles by Jansson, C.
Agricola
Right arrow Articles by Borén, M.
Right arrow Articles by Jansson, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received March 14, 2006
Accepted July 25, 2006

RESEARCH PAPER

Developmental regulation of a VEIDase caspase-like proteolytic activity in barley caryopsis

Mats Borén 1, Anna-Stina Höglund 1, Peter Bozhkov 1, and Christer Jansson 1 *

1 Department of Plant Biology and Forestry Genetics, Swedish University of Agricultural Sciences, PO Box 7080, SE-75007, Uppsala, Sweden

* To whom correspondence should be addressed.
Christer Jansson, E-mail: christer.jansson{at}vbsg.slu.se


  Abstract

Caspases are essential in animal programmed cell death both as initiator and executioner proteases. Plants do not have close caspase homologues, but several instances of caspase-like proteolytic activity have been demonstrated in connection with programmed cell death in plants. It was asked if caspase-like proteases are involved during development of the barley caryopsis. The presence of a caspase-6-like proteolytic activity that preferentially cleaved the sequence VEID was demonstrated. A range of protease inhibitors was tested and only caspase-specific inhibitors showed major inhibitory effects. The profile of VEIDase activity in developing starchy endosperm, embryo, and whole caryopsis was measured and showed a general trend of higher activity in young, rapidly developing tissues. The VEIDase activity was localized in vivo to vesicles, shown to be autophagosomes, in randomly distributed cells of the starchy endosperm. The VEIDase activity detected in barley caryopses is similar to activities described previously in mammals, spruce, yeast, and thale cress. In mammals, spruce, and yeast, VEIDase activity has been shown to be positively correlated with the occurrence of programmed cell death. Several manifestations of programmed cell death exist in developing barley caryopsis, indicating a connection between VEIDase activity and developmental programmed cell death in barley.

Keywords: Barley; caspase-like activity; programmed cell death; starchy endosperm; VEIDase.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol PlantHome page
M. Bosch, N. S. Poulter, S. Vatovec, and V. E. Franklin-Tong
Initiation of Programmed Cell Death in Self-Incompatibility: Role for Cytoskeleton Modifications and Several Caspase-Like Activities
Mol Plant, November 1, 2008; 1(6): 879 - 887.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Bonneau, Y. Ge, G. E. Drury, and P. Gallois
What happened to plant caspases?
J. Exp. Bot., February 13, 2008; (2008) erm352v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Bosch and V. E. Franklin-Tong
Self-incompatibility in Papaver: signalling to trigger PCD in incompatible pollen
J. Exp. Bot., February 1, 2008; 59(3): 481 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Bosch and V. E. Franklin-Tong
Temporal and spatial activation of caspase-like enzymes induced by self-incompatibility in Papaver pollen
PNAS, November 13, 2007; 104(46): 18327 - 18332.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.