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JXB Advance Access published online on September 27, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl139
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 17, 2006
Accepted August 2, 2006

FOCUS PAPER

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry: a technique to map plant metabolites within tissues at high spatial resolution

M. M. Burrell 1 *, C. J. Earnshaw 2, and M. R. Clench 2

1 Department of Animal and Plant Sciences, Sheffield University, Western Bank, Sheffield S10 2TN, UK
2 Biomedical Research Centre, Sheffield Hallam University, Howard Street, Sheffield S1 1WB, UK

* To whom correspondence should be addressed.
M. M. Burrell, E-mail: m.burrell{at}sheffield.ac.uk


  Abstract

Imaging Matrix Assisted Laser Desorption Ionization Mass Spectrometry provides a new and powerful tool to analyse the distribution of metabolites within plant tissues. The two matrices {alpha}-cyano-4-hydroxycinnamic acid ({alpha}-CHCA) and 9-aminoacridine provide a useful combination that allows the measurement of amino acids, sugars, and phosphorylated metabolites. Results are presented showing that representatives of these metabolites are unevenly distributed in wheat seeds at different stages of development and under temperature stress.

Keywords: 9-aminoacridine; CHCA; I-MALDI; mass spectrometry; metabolites; phosphorylated metabolites; plants; sugars; wheat.
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