Skip Navigation



JXB Advance Access published online on October 9, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl149
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/14/3837    most recent
erl149v1
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Nogués, S.
Right arrow Articles by Cornic, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nogués, S.
Right arrow Articles by Cornic, G.
Agricola
Right arrow Articles by Nogués, S.
Right arrow Articles by Cornic, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received April 5, 2006
Accepted August 2, 2006

RESEARCH PAPER

Respiratory carbon metabolism in the high mountain plant species Ranunculus glacialis

Salvador Nogués 1 *, Guillaume Tcherkez 2, Peter Streb 2, Antoni Pardo 3, Florence Baptist 4, Richard Bligny 5, Jaleh Ghashghaie 2, and Gabriel Cornic 2

1 Station Alpine Joseph Fourier, UMS UJF CNRS 2925-Col du Lautaret, 05480 Villar d'Arène, France; Departament de Biologia Vegetal, Universitat de Barcelona, 645 Diagonal Av, 08028 Barcelona, Spain; Laboratoire d'Ecologie, Systématique et Evolution, CNRS UMR 8079, IFR 87, Université Paris XI, 91405 Orsay Cedex, France
2 Station Alpine Joseph Fourier, UMS UJF CNRS 2925-Col du Lautaret, 05480 Villar d'Arène, France; Laboratoire d'Ecologie, Systématique et Evolution, CNRS UMR 8079, IFR 87, Université Paris XI, 91405 Orsay Cedex, France
3 Station Alpine Joseph Fourier, UMS UJF CNRS 2925-Col du Lautaret, 05480 Villar d'Arène, France; Departament de Biologia Vegetal, Universitat de Barcelona, 645 Diagonal Av, 08028 Barcelona, Spain
4 Station Alpine Joseph Fourier, UMS UJF CNRS 2925-Col du Lautaret, 05480 Villar d'Arène, France; Laboratoire d'Ecologie Alpine, CNRS Université Joseph Fourier BP 53, 38041 Grenoble Cedex 9, France
5 Station Alpine Joseph Fourier, UMS UJF CNRS 2925-Col du Lautaret, 05480 Villar d'Arène, France; Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche 5168, Commissariat à l'Energie Atomique-Grenoble, 38054 Grenoble Cedex 9, France

* To whom correspondence should be addressed.
Salvador Nogués, E-mail: salvador.nogues{at}ub.edu


  Abstract

Very little is known about the primary carbon metabolism of the high mountain plant Ranunculus glacialis. It is a species with C3 photosynthesis, but with exceptionally high malate content in its leaves, the biological significance of which remains unclear. 13C/12C-isotope ratio mass spectrometry (IRMS) and 13C-nuclear magnetic resonance (NMR) labelling were used to study the carbon metabolism of R. glacialis, paying special attention to respiration. Although leaf dark respiration was high, the temperature response had a Q10 of 2, and the respiratory quotient (CO2 produced divided by O2 consumed) was nearly 1, indicating that the respiratory pool is comprised of carbohydrates. Malate, which may be a large carbon substrate, was not respired. However, when CO2 fixed by photosynthesis was labelled, little labelling of the CO2 subsequently respired in the dark was detected, indicating that: (i) most of the carbon recently assimilated during photosynthesis is not respired in the dark; and (ii) the carbon used for respiration originates from (unlabelled) reserves. This is the first demonstration of such a low metabolic coupling of assimilated and respired carbon in leaves. The biological significance of the uncoupling between assimilation and respiration is discussed.

Keywords: Carbon isotopic labelling; malate; photosynthesis; Ranunculus glacialis; respiration.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
F. Baptist, G. Tcherkez, S. Aubert, J.-Y. Pontailler, P. Choler, and S. Nogues
13C and 15N allocations of two alpine species from early and late snowmelt locations reflect their different growth strategies
J. Exp. Bot., July 1, 2009; 60(9): 2725 - 2735.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Gomez-Casanovas, E. Blanc-Betes, M. A. Gonzalez-Meler, and J. Azcon-Bieto
Changes in Respiratory Mitochondrial Machinery and Cytochrome and Alternative Pathway Activities in Response to Energy Demand Underlie the Acclimation of Respiration to Elevated CO2 in the Invasive Opuntia ficus-indica
Plant Physiology, September 1, 2007; 145(1): 49 - 61.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.