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JXB Advance Access published online on October 10, 2006
Journal of Experimental Botany, doi:10.1093/jxb/erl151
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© The Author [2006]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Received May 26, 2006
Accepted August 1, 2006

RESEARCH PAPER

Differential expression within the LOX gene family in ripening kiwifruit

Bo Zhang 1, Kunsong Chen 1 *, Judith Bowen 2, Andrew Allan 2, Richard Espley 2, Sakuntala Karunairetnam 2, and Ian Ferguson 2

1 Laboratory of Fruit Molecular Physiology and Biotechnology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Biotechnology, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China
2 The Horticulture and Food Research Institute of New Zealand, Private Bag 92169, Auckland, New Zealand

* To whom correspondence should be addressed.
Kunsong Chen, E-mail: akun{at}zju.edu.cn


  Abstract

Real-time quantitative PCR was used to study lipoxygenase (LOX) gene expression patterns in kiwifruit (Actinidia deliciosa [A. Chev.] C.F. Liang et A.R. Ferguson var. deliciosa cv. Hayward) during fruit ripening, and in response to ethylene and low temperature during post-harvest storage. Six LOX genes were identified and cloned from a kiwifruit EST database. All were expressed in vegetative tissues and in the fruit. Expression of AdLox1 and AdLox5 increased markedly as fruit developed to the climacteric stage and were up-regulated by ethylene treatment, following a similar pattern to LOX enzyme activity. By contrast, AdLox2, AdLox3, and AdLox4 transcripts were negatively associated with ethylene accumulation, and ethylene application enhanced the decline in transcript levels. Transcripts of AdLox6 declined with fruit ripening. The fruit showed no ripening changes at low temperature, where transcripts of AdLox1 and AdLox6 were slightly induced about 72 h after harvest, suggesting an adaptive response to low temperature. Transient expression of the ethylene-responsive AdLox1 gene in tobacco leaves led to significant degradation of chlorophyll and promoted tissue senescence, whereas AdLox2 had no such effect. The results showed that the six LOX genes were differentially regulated during kiwifruit ripening and senescence, forming two groups, one active in ripening and responsive to ethylene and the other more constitutively expressed. The possible roles of individual LOX isoforms in kiwifruit are discussed.

Keywords: Ethylene; fruit ripening; gene expression; kiwifruit; lipoxygenase; low temperature; senescence.
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